別品名 |
HRP, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
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標識物 |
Horseradish Peroxidase
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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非交差(吸収処理)種 |
Human Mouse Rat
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Accession No.(Gene/Protein) |
Q9U6Y8
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Gene Symbol |
DsRed
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Tag情報 |
RFP
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添加剤 |
Azide Free
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参考文献 |
[Pub Med ID]31619971
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot of RFP recombinant protein detected with Rockland's unconjugated polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein. Rockland's polyclonal anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the unconjugated primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye?800 conjugated Goat-a-Rabbit IgG [H&L] MX (611-132-122). IRDye?800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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Western blot of RFP recombinant protein detected with Rockland's unconjugated polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein. Rockland's polyclonal anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the unconjugated primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye?800 conjugated Goat-a-Rabbit IgG [H&L] MX (611-132-122). IRDye?800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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メーカー |
品番 |
包装 |
RKL
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600-403-379
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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