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Western blot using Rockland's affinity purified anti-PTEN-P1 antibody shows detection at ~55kDa (arrowhead) of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (p/n W09-000-364) [lane 4], HEK293 (p/n W09-000-365) [lane 5] and MCF7 (p/n W09-000-360) [lane 6]. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. The identity of lower molecular weight bands in lane 4 is unknown. Briefly, each lane contains approximately 35μg of lysate. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800？ conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye？800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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Western blot using Rockland's affinity purified anti-PTEN-P1 antibody shows detection at ~55kDa (arrowhead) of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (p/n W09-000-364) [lane 4], HEK293 (p/n W09-000-365) [lane 5] and MCF7 (p/n W09-000-360) [lane 6]. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. The identity of lower molecular weight bands in lane 4 is unknown. Briefly, each lane contains approximately 35μg of lysate. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800？ conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye？800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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| 別品名 |
rabbit anti-PTEN-P1 antibody, Phosphatase and tensin homolog 2, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN, MMAC1, TEP1
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| 別包装 |
あり
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 抗原部位 |
N-terminus
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
5728
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| Accession No.(Gene/Protein) |
73765544, O43460
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| Gene Symbol |
PTEN
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| 参考文献 |
Phillips,R.J., Mestas,J., Gharaee-Kermani,M., Burdick,M.D., Sica,A., Belperio,J.A., Keane,M.P. and Strieter,R.M. (2005) Epidermal growth factor and hypoxia-induced expression of CXC chemokine receptor 4 on non-small cell lung cancer cells is regulated by the phosphatidylinositol 3-kinase/PTEN/AKT/mammalian target of rapamycin signaling pathway and activation of hypoxia inducible factor-1alpha. J. Biol. Chem. 280 (23), 22473-22481. Baak,J.P., Van Diermen,B., Steinbakk,A., Janssen,E., Skaland,I., Mutter,G.L., Fiane,B. and Lovslett,K. (2005) Lack of PTEN expression in endometrial intraepithelial neoplasia is correlated with cancer progression. Hum. Pathol. 36 (5), 555-561. Steck,P.A., Pershouse,M.A., Jasser,S.A., Yung,W.K., Lin,H., Ligon,A.H., Langford,L.A., Baumgard,M.L., Hattier,T., Davis,T., Frye,C., Hu,R., Swedlund,B., Teng,D.H. and Tavtigian,S.V. (1997) Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat. Genet. 15 (4), 356-362.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-A90S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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