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Immunohistochemistry of ABCB5 antibody. Tissue: human liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: ABCB5 at 1:200. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Western blot using Rockland's affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight. Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD.
Immunohistochemistry of Rabbit anti ABCB5 antibody. Tissue: human breast carcinoma at pH6. (left, neg control) (right, 20X, 40X). Fixation: formalin fixed paraffin embedded. Primary antibody: ABCB5 antibody at 10 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ABCB5 is cytoplasmic. Staining: ABCB5 as precipitated brown signal with hematoxylin purple nuclear counterstain.
Immunohistochemistry of Rabbit anti ABCB5 antibody. Tissue: human breast carcinoma at pH9. (20X, 40X) Fixation: formalin fixed paraffin embedded. Primary antibody: ABCB5 antibody at 10 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ABCB5 is cytoplasmic. Staining: ABCB5 as precipitated brown signal.
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Immunohistochemistry of ABCB5 antibody. Tissue: human liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: ABCB5 at 1:200. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
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| 別品名 |
rabbit anti-ABCB5 Antibody, ABCB 5, ABCB-5, ATP binding cassette sub family B (MDR/TAP) member 5 antibody, ATP binding cassette sub family B member 5 antibody, EST422562 antibody, P glycoprotein ABCB5 antibody
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
340273
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| Accession No.(Gene/Protein) |
56849536, Q2M3G0
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| Gene Symbol |
ABCB5
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| 参考文献 |
[Pub Med ID]20729912
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-A77
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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