| 別品名 |
170 kDa melanoma membrane bound gelatinase antibody, DPPIV antibody, Integral membrane serine protease antibody, FAPA antibody, Seprase antibody
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| 種由来 |
Mouse
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 交差種 |
Mouse
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| GENE ID |
14089
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| Accession No.(Gene/Protein) |
P97321
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| Gene Symbol |
FAP
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Cheng,J.D., Valianou,M., Canutescu,A.A., Jaffe,E.K., Lee,H.O., Wang,H., Lai,J.H., Bachovchin,W.W. and Weiner,L.M. (2005) Abrogation of fibroblast activation protein enzymatic activity attenuates tumor growth. Mol. Cancer Ther. 4 (3), 351-360. Ramirez-Montagut,T., Blachere,N.E., Sviderskaya,E.V., Bennett,D.C., Rettig,W.J., Garin-Chesa,P. and Houghton,A.N. (2004) FAPalpha, a surface peptidase expressed during wound healing, is a tumor suppressor. Oncogene 23 (32), 5435-5446. Niedermeyer,J., Garin-Chesa,P., Kriz,M., Hilberg,F., Mueller,E., Bamberger,U., Rettig,W.J. and Schnapp,A. (2001) Expression of the fibroblast activation protein during mouse embryo development. Int. J. Dev. Biol. 45 (2), 445-447.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
ELISA results of purified Rabbit anti Fibroblast Activation Protein (FAP) Antibody tested against BSA conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611 103 122) and TMB ELISA Peroxidase Substrate (p/n TMBE 1000).
Western blot using Rockland's affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). ？Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).？ The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000. ？Personal communication, S.Kim, NCI, Bethesda, MD.
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ELISA results of purified Rabbit anti Fibroblast Activation Protein (FAP) Antibody tested against BSA conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611 103 122) and TMB ELISA Peroxidase Substrate (p/n TMBE 1000).
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| メーカー |
品番 |
包装 |
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RKL
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600-401-A73
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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