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Western blot using Rockland's affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).？ The identity of the higher and lower molecular weight bands is unknown.？ The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6.？ Primary antibody was used at 1:1000. Personal communication, J.Lee, NCI, Bethesda, MD.
Western blot using Rockland's affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used. Personal communication, J.Lee, NCI, Bethesda, MD.
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Western blot using Rockland's affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).？ The identity of the higher and lower molecular weight bands is unknown.？ The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6.？ Primary antibody was used at 1:1000. Personal communication, J.Lee, NCI, Bethesda, MD.
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| 別品名 |
rabbit anti-Eif3s6/Int6 Antibody, rabbit anti-Eif3s6 Antibody, rabbit anti-Int6 Antibody, eIF3e antibody, Eukaryotic translation initiation factor 3 subunit 6 antibody, INT-6 antibody, Viral integration site protein INT 6 antibody, Mammary tumor-associated protein INT-6, MMTV integration site 6, Eukaryotic translation initiation factor 3 subunit E
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| 交差種 |
Human
Mouse
Monkey
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 抗原部位 |
C-terminus
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
16341
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| Accession No.(Gene/Protein) |
45476573, P60229
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| Gene Symbol |
Eif3e
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| 参考文献 |
Buttitta F, Martella C, Barassi F, Felicioni L, Salvatore S, Rosini S, D'Antuono T, Chella A, Mucilli F, Sacco R, Mezzetti A, Cuccurullo F, Callahan R, Marchetti A. (2005) Int6 expression can predict survival in early-stage non-small cell lung cancer patients. Clin Cancer Res. 11(9):3198-204. Lowther,W., Wiley,K., Smith,G.H. and Callahan,R. (2005) A new common integration site, Int7, for the mouse mammary tumor virus in mouse mammary tumors identifies a gene whose product has furin-like and thrombospondin-like sequences. J. Virol. 79 (15), 10093-10096. Bandyopadhyay,A., Matsumoto,T. and Maitra,U. (2000) Fission yeast Int6 is not essential for global translation initiation, but deletion of int6(+) causes hypersensitivity to caffeine and affects spore formation. Mol. Biol. Cell 11 (11), 4005-4018.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-A62
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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