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Western Blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 μg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, ~35 kDa
Western Blot of Anti-C/EBPd Antibody. Lane 1: untreated wild-type (WT) cells. Lane 2: WT cells treated 3 h with LPS. Lane 3: C/EBPd-knock-out cells treated 3H with LPS. Load: 35 μg per lane. Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C. Blocking: buffer TBST. ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, ~35 kDa.
Identification of the SIAH2 interaction domain in C/EBPδ. (A) Six different C/EBPδ-C/EBPβ chimeric proteins, as shown in the schematic and the full-length proteins, were cotransfected with Flag-SIAH2 in MCF-10A cells. Immunoprecipitates with anti-Flag antibody and input were analyzed with a pan-C/EBP and anti-Flag antibody for SIAH2. Cells had been treated with MG132 to stabilize C/EBPδ. TA, transactivation domain; BR, basic region; LZ, leucine zipper. (B) Schematic of the sequence of human C/EBPδ and C/EBPβ aligned with the consensus for SIAH2 interaction domains and two point mutations in C/EBPδ (underlined amino acids match the consensus motif). Immunoprecipitates with anti-Flag antibody and input were analyzed with antibodies specific for C/EBPβ and C/EBPδ and with anti-Flag to detect SIAH2. Cells had been treated with MG132 to stabilize C/EBPδ. (C) Western analysis of MCF-10A cells after transfection with the indicated C/EBPδ expression constructs and Flag-SIAH2. Fig 5. PMID:？22037769
(C) C/EBPδ is induced by hypoxia？in vivo. Immunohistochemistry of parallel sections of an MMTV-Neu mammary tumour stained with anti-hypoxyprobe and anti-C/EBPδ antibodies. Original magnification: × 400.？Fig 1. PMID:？21076392
b)？C/EBPδ protein increases 12.2-fold (p？<？0.0001) in pcDNA3-Cebpd cerebellar granular cultures.？ C/EBPδ protein is assessed in cerebellar granular cultures by western blot using β-actin as the normalizing protein. **p？<？0.01 and ***p？<？0.001, using Student’s？t-test. Fig 2. PMID:？31209363
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Western Blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 μg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, ~35 kDa
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| 別品名 |
rabbit anti-CEBP Delta Antibody, C/EBP related protein 3 antibody, CCAAT/enhancer binding protein delta antibody, CEBP D antibody, CEBPD antibody, CELF antibody, Crp 3 antibody, Crp3 antibody, NF IL6 beta antibody, Nuclear factor NF IL6 beta antibody
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| 別包装 |
あり
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| 交差種 |
Mouse
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 抗原部位 |
N-terminus
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
12609
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| Accession No.(Gene/Protein) |
110347410, Q00322
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| Gene Symbol |
Cebpd
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| 参考文献 |
[[Cosmo Reference Collection]][Pub Med ID]31209363,21076392,22037769
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-A61
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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