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Tat does not induce phosphorylation of CD127 at Y449. Primary human CD8 T cells were treated with either Tat (10？μg/mL) for 12？h, IL-7 (10？ng/mL) for 10？min, or left in medium alone. Cells were then lysed and analyzed by western blot for CD127 or CD127 phosphorylated at Y449. Blots were also probed for β-actin as a loading control. (A) Representative blots. (B) Bands were quantified via densitometry. Relative density of CD127 pY449 normalized to whole CD127 is shown. Error bars represent standard error of the mean (±). Statistical analysis was performed using a paired two-tailed student？t-test (n=3). Fig. 1.？PMID:？27596538
Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
Western Blot and immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4°C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
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Tat does not induce phosphorylation of CD127 at Y449. Primary human CD8 T cells were treated with either Tat (10？μg/mL) for 12？h, IL-7 (10？ng/mL) for 10？min, or left in medium alone. Cells were then lysed and analyzed by western blot for CD127 or CD127 phosphorylated at Y449. Blots were also probed for β-actin as a loading control. (A) Representative blots. (B) Bands were quantified via densitometry. Relative density of CD127 pY449 normalized to whole CD127 is shown. Error bars represent standard error of the mean (±). Statistical analysis was performed using a paired two-tailed student？t-test (n=3). Fig. 1.？PMID:？27596538
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| 別品名 |
rabbit anti-IL-7 Receptor alpha pY449 antibody, rabbit anti-Interleukin-7 Receptor alpha pY449 antibody, IL7R alpha antibody, IL-7R alpha, IL 7R α, Interleukin7 Receptor, Interleukin 7 receptor alpha chain antibody, Interleukin 7 receptor α antibody
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 翻訳後修飾 |
リン酸化
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| GENE ID |
16197
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| Accession No.(Gene/Protein) |
45269152, P16872
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| Gene Symbol |
IL17R
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| 参考文献 |
[[Cosmo Reference Collection]][Pub Med ID]27596538
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-A49
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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