Anti THRA  

Western blot using Rockland's affinity purified anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3). No staining is evident in lysates from mock-transfected 293 cells (lane 2). Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4). Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus. The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA. Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD.

Differential recruitment of TRβ1PV and TRα1PV to the promoter of the？C/ebpα？gene as determined by ChIP analysis. ChIP assays were performed using control 3T3-L1 (lanes 1？7), L1-β1PV (lanes 8？14), and L1-α1PV (lanes 15？21) cells after T3-induced adipogenesis on day 9. Antibodies used in the chromatin immunoprecipitation were anti-Ac-H3 antibody as a positive control (lanes 4, 11, and 18), anti-TRα1 (p/n 600-401-A38) antibody (lanes 5, 12, and 19), anti-TRβ1 (p/n 600-401-A96) antibody (lanes 6, 13, and 20), and anti-PV antibody (lanes 7, 14, and 21). The negative controls were no antibody (lanes 2, 9, and 16) as well as an irrelevant IgG (lanes 3, 10, and 17). The chromatin immunoprecipitated and recovered DNA was used as a template for PCR amplification of the receptor-binding region in the promoter of the？C/ebpα？gene. Two percent of the chromatin solution (20 μl) was used for input DNA as a control. Three separate experiments were performed, and the representative results are shown. Fig 6. PMID:？20080985

Double immunofluorescence images of expression of keratin 14 (K14, green) with TRα or TRβ (red). Images were obtained from the interfollicular epidermis (IFE) and the hair follicles (HF) of wild-type mice. The slides were counterstained with DAPI (blue) and the merged images are shown. Bars: 50 μM. Fig 3. PMID:？25254665

(C) Western blotting demonstrating protein levels of TRα and TRβ in human erythroid cells at days 9 and 15 of culture.？ Fig 1. PMID:？28864529