Anti Sipa1  

Rockland's affinity purified anti-Sipa1 antibody was used at 1.25 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Western blot using Rockland's affinity purified anti-Sipa1 antibody shows detection of over-expressed Sipa1 in lysates from mouse 3T3 cells transfected with Sipa1 (lane 1). Endogenous Sipa1 is detected in lane 2, which contains lysate from 3T3 cells mock-transfected with LacZGLB, although at a significantly reduced level compared to transfected cells. Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane. The identity of the higher and lower molecular weight bands is unknown. The band at ~130 kDa, indicated by the arrowhead, corresponds to recombinant Sipa1. Primary antibody was used at 1:1250. Personal communication, H. Yang, L. Lukes and K. Hunter, NCI, Bethesda, MD.

Immunohistochemistry of rabbit anti-Sipa1 antibody. Tissue: small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Sipa1 at 5 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sipa-1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of rabbit anti-Sipa1 antibody. Tissue: tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Sipa1 at 5 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sipa-1 as precipitated red signal with hematoxylin purple nuclear counterstain.