別品名 |
GTPase activating protein Spa 1 antibody, MGC102688 antibody, MGC17037 antibody, p130 SPA1 antibody, Signal induced proliferation associated 1 antibody
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抗原部位 |
N-terminus
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種由来 |
Mouse
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry
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免疫動物 |
Rabbit
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交差種 |
Human Mouse
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GENE ID |
20469
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Accession No.(Gene/Protein) |
AAH54824, P46062
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Gene Symbol |
SIPA1
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形状 |
滅菌済み液状品
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry of rabbit anti Sipa1 antibody. Tissue: small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti Sipa1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sipa 1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Immunohistochemistry of rabbit anti-Sipa1 antibody. Tissue: tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Sipa1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sipa-1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Rockland's affinity purified anti-Sipa1 antibody was used at 1.25 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Western blot using Rockland's affinity purified anti-Sipa1 antibody shows detection of over-expressed Sipa1 in lysates from mouse 3T3 cells transfected with Sipa1 (lane 1). Endogenous Sipa1 is detected in lane 2, which contains lysate from 3T3 cells mock-transfected with LacZGLB, although at a significantly reduced level compared to transfected cells. Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane. The identity of the higher and lower molecular weight bands is unknown. The band at ~130 kDa, indicated by the arrowhead, corresponds to recombinant Sipa1. Primary antibody was used at 1:1250. Personal communication, H. Yang, L. Lukes and K. Hunter, NCI, Bethesda, MD.
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Immunohistochemistry of rabbit anti Sipa1 antibody. Tissue: small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti Sipa1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sipa 1 as precipitated red signal with hematoxylin purple nuclear counterstain.
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メーカー |
品番 |
包装 |
RKL
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600-401-A36
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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