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Western blot using Rockland's affinity purified anti-Cripto-1 antibody shows detection of endogenous and transfected Cripto-1 from mouse and human sources. The Cripto-1 band appears above the 17.8 kDa marker. Endogenous detection is shown using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells. EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing mouse Cripto-1. NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose Cripto-1 expression. COS7 cells transfected with human Cripto-1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control. A non-specific band at 45kDa may be present in some preparations. The primary antibody was used at a 1:500 dilution. Personal communication, C. Bianco, NCI, Bethesda, MD
Examples of immunostaining showing moderate (2) Nodal intensity staining (A) and strong (3) Lefty (C) and Cripto-1 (D) staining and a negative control IgG (B) in human glioblastoma biopsy sections. The arrowhead points to a mitotic cell in the specimen stained for Cripto-1. Fig 2. PMID:？24466376
Different degrees of protein expression of Nodal, Lefty, and Cripto-1 in human glioblastomas. Nodal: scores 0 (A), 1 (B), and 2 (C). Negative control IgG (D). Lefty: scores 0 (E), 1 (F), 2 (G), and 3 (H). Cripto-1: scores 0 (I), 1 (J), 2 (K), and 3 (L). Fig 3. PMID:？24466376
Immunohistochemistry results. Representative images of immunohistochemistry staining results showing weak and strong staining for Cripto-1 in nevi. IS, index score. Fig 1. PMID:？27446436
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Western blot using Rockland's affinity purified anti-Cripto-1 antibody shows detection of endogenous and transfected Cripto-1 from mouse and human sources. The Cripto-1 band appears above the 17.8 kDa marker. Endogenous detection is shown using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells. EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing mouse Cripto-1. NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose Cripto-1 expression. COS7 cells transfected with human Cripto-1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control. A non-specific band at 45kDa may be present in some preparations. The primary antibody was used at a 1:500 dilution. Personal communication, C. Bianco, NCI, Bethesda, MD
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| 別品名 |
rabbit anti-Cripto 1 antibody, rabbit anti-Cripto-1 antibody, Cripto 1 growth factor antibody, Epidermal growth factor like cripto protein CR1 antibody, TDGF 1 antibody, Teratocarcinoma-derived growth factor 1, CRGF, TDGF1
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| 別包装 |
あり
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| 交差種 |
Human
Mouse
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
6997
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| Accession No.(Gene/Protein) |
4507425, P13385
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| Gene Symbol |
TDGF1
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| 参考文献 |
[[Cosmo Reference Collection]][Pub Med ID]20568225,31310365,24466376,27446436,27467944
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-997
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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