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Western blot using Rockland's affinity purified anti-CDK9 pT29 antibody shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29. The primary antibody was used at a 1:1000 dilution. Personal Communication, J. Brady, NCI, Bethesda, MD.
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Western blot using Rockland's affinity purified anti-CDK9 pT29 antibody shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29. The primary antibody was used at a 1:1000 dilution. Personal Communication, J. Brady, NCI, Bethesda, MD.
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| 別品名 |
rabbit anti-CDK9 pT29 antibody, Cell division protein kinase 9, Cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, Cell division cycle 2-like protein kinase 4, C-2K, Tat-associated kinase complex catalytic subunit, CDC2L4, TAK
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 翻訳後修飾 |
リン酸化
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| GENE ID |
1025
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| Accession No.(Gene/Protein) |
4502747, P50750
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| Gene Symbol |
CDK9
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| 参考文献 |
Zhou, M., Lu, H., Park, H., Wilson-Chiru, J., Linton, R. and Brady, J.N. (2006) Tax Interacts with P-TEFb in a Novel Manner To Stimulate Human T-Lymphotronic Virus Type 1 Transcription. J. Virol. 80 (10): 4781-4791. Jang, M.K., Mochizuki, K., Zhou, M., Jeong, H.-S., Brady, J.N. and Ozato, K. (2005) Bromodomain Protein Brd4 is a Positive Regulatory Component of P-TEFb and Stimulates RNA Polymerase II Dependent Transcription. Mol. Cell 19: 523-534. Liu, H. and Herrmann, C.H. (2005) Differential localization and expression of the Cdk9 42k and 55k isoforms. J. Cell. Physiol. 203 (1): 251-260. Peng, J., Zhu, Y., Milton, J.T. and Price, D.H. (1998). Identification of multiple cyclin subunits of human P-TEFb. Genes Dev. 12 (5): 755-762. Zhou, Q., Chen, D., Pierstorff, E. and Luo, K. (1998) Transcription elongation factor P-TEFb mediates Tat activation of HIV-1 transcription at multiple stages. EMBO J. 17(13): 3681-3691.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-996
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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