Anti TAF1  

Immunohistochemistry of rabbit anti-TAF1 antibody. Tissue: prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-TAF1 at 10 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TAF-1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Western blot using Rockland's affinity purified anti-TAF1 to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1. Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD.

Western blot using Rockland's affinity purified anti-TAF1 to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1. Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD.

Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). CHIP was performed on fos promoters using 5 μg of TAF1 antibody from Rockland (P/n 600-401-995) and an RNA PolII antibody. Image with data provided courtesy of Shiraz Mujtaba, Ph.D., Dept. of Structural & Chemical Biology, Mount Sinai School of Medicine.

Western Blot of Rabbit anti-TAF1 antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-363). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: A549 Lysate (p/n W09-001-372). Lane 7: LNCap Lysate (p/n W09-001-GJ9). Lane 8: MOLT-4 Lysate (p/n W09-001-GK2). Lane 9: Ramos Lysate (p/n W09-000-GK4). Lane 10: Raji Lsyate (p/n W09-001-368). Lane 11: A-172 Lysate (p/n W09-001-GL5). Lane 12: NIH/3T3 Lysate (p/n W10-000-358). Load: 35 μg per lane. Primary antibody: TAF1 antibody at 0.2ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 250kDa for TAF1.