Anti Transcription Initiation Factor TFIID Subunit 1 (TAF1)

Immunohistochemistry of rabbit anti-TAF1 antibody.
Tissue: prostate.
Fixation: formalin fixed paraffin embedded.
Antigen retrieval: not required.
Primary antibody: Anti-TAF1 at 10 μg/mL for 1 h at RT.
Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
Staining: TAF-1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Western blot using Rockland's affinity purified anti-TAF1 to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1. Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD.

Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). CHIP was performed on fos promoters using 5 μg of TAF1 antibody from Rockland (P/n 600-401-995) and an RNA PolII antibody. Image with data provided courtesy of Shiraz Mujtaba, Ph.D., Dept. of Structural & Chemical Biology, Mount Sinai School of Medicine.