Anti c-MET, phospho Tyr1349/Tyr1356

Western blot using Rockland's affinity purified anti c Met pY1349pY1356 antibody shows detection of phosphorylated c Met. Human mammary B5/589 epithelial cells were serum deprived and treated with or without HGF. Cell lysates were immunoprecipitated with the anti c Met antibody, resolved by SDS PAGE, transferred to PVDF membrane, and probed with anti c Met pY1349pY1356. Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD

Western blot using Rockland’s affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary epithelial cells (B5/589) were serum deprived and stimulated with (+) and without (-) HGF. Cell lysates were immunoprecipitated using a human anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane and probed using various antibodies. Lane 1 and 2 where probed using the anti-c-Met pY1349pY1356 antibody (#600-401-989), lane 3 and 4 where probed using an anti-phosphotyrosine antibody as phosphorylation control and lane 5 and 6 where probed using an anti-cMet antibody as a total Met loading control. Bands recognized at an apparent molecular weight of ~170 kDa are total Met, ~145 kDa phosphorylated Met beta chain in HGF +, ~150 kDa phosphorylated Met in +/- HGF, ~50kDa phosphorylated Met alpha chain. Personal communication with D. Bottaro and F. Cecchi, CCR-NCI, Bethesda, MD.