別品名 |
HGF receptor antibody, HGF/SF receptor antibody, HGFR antibody, MET antibody, Met proto oncogene tyrosine kinase antibody
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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交差種 |
Human
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翻訳後修飾 |
リン酸化
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GENE ID |
4233
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Accession No.(Gene/Protein) |
P08581
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Gene Symbol |
MET
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形状 |
滅菌済み液状品
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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Western blot using Rockland's affinity purified anti c Met pY1349pY1356 antibody shows detection of phosphorylated c Met. Human mammary B5/589 epithelial cells were serum deprived and treated with or without HGF. Cell lysates were immunoprecipitated with the anti c Met antibody, resolved by SDS PAGE, transferred to PVDF membrane, and probed with anti c Met pY1349pY1356. Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD
Western blot using Rockland’s affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary epithelial cells (B5/589) were serum deprived and stimulated with (+) and without (-) HGF. Cell lysates were immunoprecipitated using a human anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane and probed using various antibodies. Lane 1 and 2 where probed using the anti-c-Met pY1349pY1356 antibody (#600-401-989), lane 3 and 4 where probed using an anti-phosphotyrosine antibody as phosphorylation control and lane 5 and 6 where probed using an anti-cMet antibody as a total Met loading control. Bands recognized at an apparent molecular weight of ~170 kDa are total Met, ~145 kDa phosphorylated Met beta chain in HGF +, ~150 kDa phosphorylated Met in +/- HGF, ~50kDa phosphorylated Met alpha chain. Personal communication with D. Bottaro and F. Cecchi, CCR-NCI, Bethesda, MD.
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Western blot using Rockland's affinity purified anti c Met pY1349pY1356 antibody shows detection of phosphorylated c Met. Human mammary B5/589 epithelial cells were serum deprived and treated with or without HGF. Cell lysates were immunoprecipitated with the anti c Met antibody, resolved by SDS PAGE, transferred to PVDF membrane, and probed with anti c Met pY1349pY1356. Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD
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メーカー |
品番 |
包装 |
RKL
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600-401-989S
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25 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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