Anti Tamalin  

Western blot using Rockland's affinity purified anti-Tamalin to detect over-expressed Tamalin in HEK293 cells (lane 2, arrowhead). Lane 1 shows the non-transfected control. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:2,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

Mouse brain lysate was immunoprecipitated with anti-Tamalin antiserum. A blot was prepared and probed with affinity purified anti-Tamalin. Lane 1 is wild-type brain lysate; Lane 2 is Tamalin knock-out brain lysate. The membrane was probed with the primary antibody at a 1:1,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

Knockdown of endogenous tamalin inhibits the ligand-mediated endocytosis of mGluR1.？A？and？B, Western blotting (A) and quantitation of the Western blots (B), showing the efficient knock-down of endogenous tamalin by shTam and replacement of the endogenous tamalin with full-length tamalin.？Fig 1. PMID:？32376687

N-terminal domain of tamalin alone is not sufficient for the ligand-mediated endocytosis of mGluR1.？A？and？B, Western blotting (A) and quantitation of the Western blots (B), showing the effective knockdown of endogenous tamalin by shTam and expression of the N-Tam replacement construct.？Fig 2. PMID:？32376687

The C-terminal domain of tamalin alone is not sufficient for the ligand-mediated endocytosis of mGluR1.？A？and？B, Acute knockdown of endogenous tamalin and replacement of endogenous tamalin with C-Tam, as shown by Western blotting (A) and quantitation of the Western blots (B).？Fig 3. PMID:？32376687

The last 8 amino acids of tamalin play a critical role in the ligand-mediated internalization of mGluR1.？A？and？B, Western blotting (A) and quantitation of the Western blots (B), showing the knockdown of the endogenous tamalin by shTam and expression of the TamΔ8 replacement construct. G, Coimmunoprecipitation assays demonstrated that deletion of the last 8 amino acids of tamalin disrupted binding of S-SCAM to tamalin.？H, Quantitation of the coimmunoprecipitation assays.？？Fig 4. PMID:？32376687