別品名 |
AO7 antibody, E3 ubiquitin protein ligase RNF25 antibody, EC 6.3.2.- antibody, FLJ13906 antibody, Ring finger protein 25 antibody, RING finger protein AO7 antibody, RNF 25 antibody
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抗原部位 |
C-terminus
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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交差種 |
Human Mouse
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GENE ID |
64320
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Accession No.(Gene/Protein) |
Q96BH1
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Gene Symbol |
RNF25
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形状 |
滅菌済み液状品
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参考文献 |
Lorick, K. L., Jensen, J. P., Fang, S., Ong, A. M., Hatakeyama, S., and Weissman, A. M. (1999) RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Proc. Natl. Acad. Sci. USA 96, 11364-11369. Asamitsu, K., Tetsuka, T., Kanazawa, S., and Okamoto, T. (2003) RING finger protein AO7 supports NF-kB-mediated transcription by interacting with the transactivation domain of the p65 subunit. J. Biol. Chem. 278, 26879-26887.
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
1 ug of Rocklands affinity purified anti RNF25 was used in immunoprecipitation of 20 ug HEK293 cell lysate over expressing HA tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti HA tag antibody. Personal Communication, Allan Weissman, CCR NCI, Bethesda, MD.
Western blot using Rockland's affinity purified anti-RNF25 antibody shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 3 is a vector only control. The extracts were loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
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1 ug of Rocklands affinity purified anti RNF25 was used in immunoprecipitation of 20 ug HEK293 cell lysate over expressing HA tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti HA tag antibody. Personal Communication, Allan Weissman, CCR NCI, Bethesda, MD.
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メーカー |
品番 |
包装 |
RKL
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600-401-969
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100 UG
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※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
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保存温度 |
-20℃
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