| 別品名 |
AO7 antibody, E3 ubiquitin protein ligase RNF25 antibody, EC 6.3.2.- antibody, FLJ13906 antibody, Ring finger protein 25 antibody, RING finger protein AO7 antibody, RNF 25 antibody
|
| 抗原部位 |
C-terminus
|
| 種由来 |
Human
|
| 標識物 |
Unlabeled
|
| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunoprecipitation
|
| 免疫動物 |
Rabbit
|
| 交差種 |
Human
Mouse
|
| GENE ID |
64320
|
| Accession No.(Gene/Protein) |
34878787, Q96BH1
|
| Gene Symbol |
RNF25
|
| 形状 |
滅菌済み液状品
|
| その他 |
[Uniprot ID]Q96BH1
|
| 参考文献 |
Lorick, K. L., Jensen, J. P., Fang, S., Ong, A. M., Hatakeyama, S., and Weissman, A. M. (1999) RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Proc. Natl. Acad. Sci. USA 96, 11364-11369. Asamitsu, K., Tetsuka, T., Kanazawa, S., and Okamoto, T. (2003) RING finger protein AO7 supports NF-kB-mediated transcription by interacting with the transactivation domain of the p65 subunit. J. Biol. Chem. 278, 26879-26887.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot using Rockland's affinity purified anti-RNF25 antibody shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 3 is a vector only control. The extracts were loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
1 μg of Rockland's affinity purified anti-RNF25 was used in immunoprecipitation of 20 μg HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
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|
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Western blot using Rockland's affinity purified anti-RNF25 antibody shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 3 is a vector only control. The extracts were loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
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|
| メーカー |
品番 |
包装 |
|
RKL
|
600-401-969
|
100 UG
[1.0 mg/mL]
|
| 希望販売価格 |
¥89,000 ▼ キャンペーン価格 ¥61,410(〜2022/09/02) |
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
|
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