Anti PCNA

PAGE MAP (microsphere affinity proteomics) of Rabbit Anti PCNA Antibody (Catalog Number: 600 401 968, Lot Number: 32579). Antibody array western blot binding of gelfree size separated fractions of multiple lysates (solid lines) and shotgun mass spectroscopy identification (dashed lines) of the target band run in parallel correlate confirming the specificity of this antibody against PCNA. Data was provided by the Lund Johansen lab of Oslo University Hospital. For more information on PAGE MAP/IP MS identification of antibody specificity and its large scale implementation for antibody validation see Sikorski et. al., (2018) Nature Methods 15, 909 912.

Western blot using Rockland's affinity purified anti-PCNA antibody shows detection of PCNA protein. Lane 1: HEK293 whole cell extracts (p/n W09-000-365). Lane 2: Jurkat whole cell extracts (p/n W09-001-370). Approximately 25ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10,000 dilution of IRDyeR 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (p/n 611-132-122) (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Other detection systems will yield similar results.