Anti ABCB6  

Western blot using Rockland's affinity purified anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag tagged human ABCB6 protein at 104 kDa and a truncated form of the protein at 79 kDa (arrowheads). The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein. A lysate prepared from KB cells without a vector insert (CV lane) showed no reactivity with the antibody. The left panel shows a similar staining pattern using an anti-Flag^TM antibody as a positive control. The membrane was probed with the primary antibody diluted to 1:1,400. Personal Communication, Jill Paterson, CCR-NCI, Bethesda, MD.

The protein expressions of PEPT1, ABCB6, and ABCG2 were detected by Western blotting.No cells were incubated with 1？mM ALA. (a) Expression at different cell density in the 2D culture. Fig 5. PMID:？27857072

The protein expressions of PEPT1, ABCB6, and ABCG2 were detected by Western blotting.No cells were incubated with 1？mM ALA. (b) Expression with different sizes of spheroids. Fig 5. PMID:？27857072

Porphyrin metabolism changed in cancer cells on addition of methotrexate (MTX) or cycloheximide (CHX). MTX was cultured with 10？μM and CHX was cultured with 10？μg/ml for 48？h. (a) Protein expression was detected by Western blotting. Fig 6. PMID:？27857072

(B) Protein expression levels of porphyrin biosynthesis-related transporters PEPT1, ABCB6, and ABCG2. ？-actin was used as the loading control.？Fig 3. PMID:？40064433

Coimmunoprecipitation of ABCB5β-ABCB6 and ABCB5β-ABCB9 demonstrates the presence of these heterodimers in Mel JuSo and UACC-257？cells.？The Mel JuSo (A) or UACC-257 (B) proteins were immunoprecipitated (IP) with either an anti-ABCB5, anti-ABCB6, or anti-ABCB9 antibody. The precipitated proteins were revealed by Western blotting after SDS-PAGE using the corresponding antibody indicated on the？right side？of each blot. Fifteen micrograms of total proteins from the starting cell lysate were loaded in the first lane, while the total IP eluate was loaded on the gel. ABCB5β was expressed in High-Five insect cells, and total membrane proteins were prepared and loaded on the gel (High5 ABCB5β) as a complementary molecular weight marker when using anti-ABCB5. An isotype control was performed to determine the specificity of the signal obtained in Western blot. Fig 3. PMID:？38145744

Expression of ABCB5β, ABCB6, ABCB5β_P-gp linker_ABCB6, ABCB6_P-gp linker_ABCB5β, ABCB9, ABCB5β_P-gp linker_ABCB9, and ABCB9_P-gp linker_ABCB5β in High-Five insect cells.？Total membranes vesicles prepared from High-Five cells infected with baculovirus containing either ABCB5β, ABCB6, ABCB9, ABCB5β_P-gp linker_ABCB6, ABCB6_P-gp linker_ABCB5β, ABCB5β_P-gp linker_ABCB9, ABCB9_P-gp linker_ABCB5β and their corresponding EQ mutants were subjected to SDS-PAGE, followed by Western blotting with anti-ABCB5 and anti-ABCB6 antibodies (B).？Bands of interest are highlighted by？black arrows. Fig 5. PMID:？38145744