Anti MCM2  

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別品名 BM28 antibody, CCNL 1 antibody, CCNL1 antibody, CDC like 1 antibody, Minichromosome maintenance complex component 2 antibody
抗原部位 a.a.21-31, N-terminus
種由来 Human
標識物 Unlabeled
精製度 Affinity Purified
適用 Western Blot
Enzyme Linked Immunosorbent Assay
免疫動物 Rabbit
交差種 Human
Saccharomyces cerevisiae
GENE ID 4171
Accession No.(Gene/Protein) 33356547, P49736
Gene Symbol MCM2
形状 滅菌済み液状品
その他 [Uniprot ID]P49736
参考文献 Shukla,A., Navadgi,V.M., Mallikarjuna,K. and Rao,B.J. (2005) Interaction of hRad51 and hRad52 with MCM complex: a cross-talk between recombination and replication proteins. Biochem. Biophys. Res. Commun. 329 (4), 1240-1245. Tsvetkov,L. and Stern,D.F. (2005) Interaction of chromatin-associated Plk1 and Mcm7. J. Biol. Chem. 280 (12), 11943-11947. Todorov,I.T., Pepperkok,R., Philipova,R.N., Kearsey,S.E., Ansorge,W. and Werner,D. (1994) A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division. J. Cell. Sci. 107 (PT 1), 253-265.


Western blot using Rockland's Affinity Purified anti-MCM2 antibody shows detection of both phosphorylated and unphosphorylated MCM2 present in nuclear extracts from elutriated human cells (MO59K/K562). The MCM2 protein is phosphorylated after initiation of DNA replication, therefore, the protein is unphosphorylated in early S phase, and gradually becomes phosphorylated throughout S phase. In G2/M, all MCM2 is phosphorylated. Panel A shows western blot results for lysates were prepared from asynchronous cells (lane 1), cells arrested in early S with aphidicolin (lane2), and cells arrested in mitosis with nocodazole (lane 3). Panel B shows a schematic diagram of bands representing phosphorylated and unphosphorylated MCM2 present in these preparations. Asynchronous cells contain a doublet of both forms. Aphidicolin treated cells contain only unphosphorylated MCM2 and nocodozole treatment results in only phosphorylated MCM2 detected in the lysate. The phosphorylated band migrates faster than the unphosphorylated form and is seen as the lower band. There is a clear switch from the unphosphorylated form in the center lane, to the phosphorylated form in the third lane, confirming recognition of both forms of MCM2 by this antibody. The primary antibody was diluted 1:40 for this experiment. Personal Communication, Jennifer Seiler, NIH, CCR, Bethesda, MD.

メーカー 品番 包装
RKL 600-401-922 100 UG
[0.98 mg/mL]
希望販売価格 ¥89,000


当社在庫 なし
納期目安 約10日
保存温度 -20℃




メーカー名 Rockland Immunochemicals, Inc.
略号 RKL