|
※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot using Rockland's affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells in Lane 1. VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein ~97kDa. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
|
|
|
|
Western blot using Rockland's affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells in Lane 1. VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein ~97kDa. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
|
|
| 別品名 |
rabbit anti-SPANX antibody, SPANXC, SPANX-C, Sperm protein associated with the nucleus on the X chromosome C, Cancer-testis-associated protein CTp11 antibody, Cancer/testis antigen 11.3, CTP11 antibody, Nuclear associated protein SPAN Xc antibody, SPANX family member C antibody
|
| 交差種 |
Human
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
|
| 免疫動物 |
Rabbit
|
| 抗原部位 |
a.a.31-47, N-terminus
|
| 標識物 |
Unlabeled
|
| 精製度 |
Affinity Purified
|
| GENE ID |
64663
|
| Accession No.(Gene/Protein) |
57471638, Q9NY87
|
| Gene Symbol |
SPANXC
|
| 参考文献 |
Kouprina N, Mullokandov M, Rogozin I, Collins K, Solomon G, Risinger J,Koonin E, Barrett JC. Larionov V. (2004) The SPANX gene family of cancer-testis specific antigens: rapid evolution, an unusual case of positive selection and amplification in African great apes and hominids. Proc Natl Acad Sci USA 101: 3077-3082. Wang Z, Zhang Y, Liu H, Salati E, Chiriva-Internati M, Lim SH. (2003) Gene expression and immunologic consequence of SPAN-Xb in myeloma and other hematologic malignancies. Blood 101: 955-960. Westbrook VA, Diekman AB, Klotz KL, Khole VV, von Kap-Herr C, Golden WL, Eddy RL, Shows TB, Stoler MH, Lee CY, Flickinger CJ, Herr JC. (2000)Spermatid-specific expression of the novel X-linked gene product SPAN-X localized to the nucleus of human spermatozoa. Biol Reprod 63: 469-481.
|
| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
|
|
| メーカー |
品番 |
包装 |
|
RKL
|
600-401-916
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
|
|
