Anti ASAP1, phospho Tyr782

Immunofluorescent microscopy using Rockland's Affinity Purified anti-ASAP1 pY782 antibody shows detection of phosphorylated ASAP1 present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.

Rockland's affinity purified anti-ASAP1 pY782 antibody was used at 20 μg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 4X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpan Biosciences, Seattle, WA.