Anti EGFR

Western blot using Rockland's Affinity Purified anti EGFR antibody shows detection of a band at ~170 kDa corresponding to human EGFR (arrowhead). Lane 1: unstimulated A431 whole cell lysate (p/n W09 000 361). Lane 2: EGF (50 ng/ml for 15 min) stimulated A431 whole cell lysates (p/n W09 000 362). Approximately 30ug of lysate was separated on a 4 20% Tris Glycine gel by SDS PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt a Rabbit IgG [H&L] MX (p/n 611 132 122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI COR. IRDye is a trademark of LI COR, Inc. Other detection systems will yield similar results.