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Western blot using Rockland's Affinity Purified anti-mTOR antibody shows detection of a band ~245 kDa corresponding to human mTor (arrowhead).？？ Approximately 30μg of HEK293 cell lysate (p/n W09-000-365) was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose.？ After blocking, the membrane was probed with the primary antibody diluted to 1:650 for 2h at RT.？ The membrane was washed and reacted with a 1:10,000 dilution of IRDye？800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature. IRDye？800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.？ Other detection systems will yield similar results.
Diagram of Metabolic and autocrine regulation of the mTOR pathway by b-cells.
ELISA results of purified Rabbit anti-mTOR Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1μg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel (p/n MB-066), Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-103-122) and TMB ELISA Peroxidase Substrate (p/n TMBE-1000).
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Western blot using Rockland's Affinity Purified anti-mTOR antibody shows detection of a band ~245 kDa corresponding to human mTor (arrowhead).？？ Approximately 30μg of HEK293 cell lysate (p/n W09-000-365) was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose.？ After blocking, the membrane was probed with the primary antibody diluted to 1:650 for 2h at RT.？ The membrane was washed and reacted with a 1:10,000 dilution of IRDye？800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature. IRDye？800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.？ Other detection systems will yield similar results.
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| 別品名 |
rabbit anti-mTOR pS2448 antibody, FKBP12 rapamycin complex associated protein antibody, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 抗原部位 |
a.a.2440-2457
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
2475
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| Accession No.(Gene/Protein) |
1169735, P42345
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| Gene Symbol |
MTOR
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| 参考文献 |
[Pub Med ID]36428503
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| メーカー |
品番 |
包装 |
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RKL
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600-401-897
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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