Anti mTOR

Diagram of Metabolic and autocrine regulation of the mTOR pathway by b cells.

ELISA results of purified Rabbit anti-mTOR Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1μg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel (p/n MB-066), Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-103-122) and TMB ELISA Peroxidase Substrate (p/n TMBE-1000).

Western blot using Rockland's Affinity Purified anti-mTOR antibody shows detection of a band ~245 kDa corresponding to human mTor (arrowhead).？？ Approximately 30ug of HEK293 cell lysate (p/n W09-000-365) was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose.？ After blocking, the membrane was probed with the primary antibody diluted to 1:650 for 2h at RT.？ The membrane was washed and reacted with a 1:10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature. IRDyeTM800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.？ Other detection systems will yield similar results.