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Immunoprecipitation (right) and western blot (left) using Rockland's Affinity Purified anti-Cbl-c antibody shows detection of a predominant band at ~52 kDa corresponding to Cbl-c. Lysates used are from HEK293T cells transfected with empty vector or with Cbl-c and western blotting (left panel). The predicted size of Cbl-c is 52 kDa. Size markers in kDa are shown to the left of the panel. The (right panel) shows immunoprecipitation with Rabbit anti-Cbl-c followed by western blotting using a Goat anti-Cbl-c antibody. Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.
Rockland's affinity purified anti-Cbl-c antibody was used at 5 μg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining of human pancreatic acinar epithelium at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Rockland's Affinity Purified anti-Cbl-c antibody shows strong nuclear and cytoplasmic staining of cells in tubuli in human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
Tumor growth of HCC827 cells expressing control and shRNAs in xenograft models. The harvested tumors were subjected to IHC staining (E).Representative (left) and quantitative (right) graphs of Ki67, cleaved caspase-3 (cCasp3), and CD31-stained tumor section. Scale bar, 100 μm. Ki-67 and cleaved caspase-3 are expressed as percentage of positive cells per field and CD31 is expressed as positive vessels per field (×400) from eight fields derived from four sections per group (*,？P？< 0.05; **,？P？< 0.01; ***,？P？< 0.001). ns, nonsignificant. Fig 2. PMID:？29945960
The correlation of upregulated CBLC and pEGFR in LUAD.？A,？The representative IHC staining images and the correlation of CBLC and pEGFR in LUAD. The correlation between CBLC and pEGFR expression in 30 cases of LUAD was evaluated through Fisher exact test (P？= 0.0656). Scale bars, 100 μm.？Fig 7. PMID:？29945960
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Immunoprecipitation (right) and western blot (left) using Rockland's Affinity Purified anti-Cbl-c antibody shows detection of a predominant band at ~52 kDa corresponding to Cbl-c. Lysates used are from HEK293T cells transfected with empty vector or with Cbl-c and western blotting (left panel). The predicted size of Cbl-c is 52 kDa. Size markers in kDa are shown to the left of the panel. The (right panel) shows immunoprecipitation with Rabbit anti-Cbl-c followed by western blotting using a Goat anti-Cbl-c antibody. Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.
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| 別品名 |
rabbit anti-Cbl-c Antibody, E3 ubiquitin-protein ligase CBL-C, RING finger protein 57, RING-type E3 ubiquitin transferase CBL-C, SH3-binding protein CBL-3, SH3-binding protein CBL-C, Signal transduction protein CBL-C, CBLC, CBL3, RNF57
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immunoprecipitation
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| 免疫動物 |
Rabbit
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| 抗原部位 |
a.a.444-458
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
23624
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| Accession No.(Gene/Protein) |
125987803, Q9ULV8
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| Gene Symbol |
CBLC
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| 参考文献 |
[[Cosmo Reference Collection]][Pub Med ID]29945960,35149839
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-889
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100 UG
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※表示価格について
| 販売状況 |
長期B.O、納期未定
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| 当社在庫 |
なし
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| 納期目安 |
納期未定
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| 保存温度 |
-２０℃
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