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Immunofluorescence Microscopy of Rabbit Anti HDAC 1 antibody. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: HDAC 1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC 1 is nuclear. Staining: HDAC 1 was used with Atto 425 (shown in red). Anti Keratin monoclonal antibody was used with Dylight 488 (shown in green) to detect Keratin. Data was collected on a STED CW TCS SP5 Confocal system (Leica Microsystems).
Immunohistochemistry of Rabbit Anti-HDAC-1 Antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 precipitated purple with blue counterstain. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.
Immunohistochemistry of Rabbit Anti-HDAC-1 Antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 as brown color indicates presence of protein, blue color shows cell nuclei.
Immunofluorescence Microscopy of Rabbit anti-HDAC1 Antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum. Primary antibody: HDAC1 antibody at 4 ug/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 0.2 ug/mL for 45 min at RT. Localization: HDAC1 is nuclear. Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.
Western Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: 293 whole cell lysate (p/n W09-000-365). Load: 35 ug per lane. Primary antibody: HDAC-1 antibody at 1:3,500 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO (p/n B501-0500) overnight at 4C. Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.
Western Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane. Primary antibody: HDAC-1 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55kDa for HDAC-1.
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