| 別品名 |
Calcium/calmodulin dependent protein kinase II alpha antibody, CaM kinase II alpha antibody, CaM kinase II alpha chain antibody, CaM kinase II alpha subunit antibody, CaM kinase II antibody, CaM kinase II beta antibody
|
| 抗原部位 |
a.a.6-23, N-terminus
|
| 種由来 |
Human
|
| 標識物 |
Unlabeled
|
| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
|
| 免疫動物 |
Rabbit
|
| 交差種 |
Mouse
|
| GENE ID |
815
|
| Accession No.(Gene/Protein) |
NP_741960, Q9UQM7
|
| Gene Symbol |
CAMK2A
|
| 形状 |
滅菌済み液状品
|
| 参考文献 |
Lin,C.R., Kapiloff,M.S., Durgerian,S., Tatemoto,K., Russo,A.F., Hanson,P., Schulman,H. and Rosenfeld,M.G. (1987) Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase. Proc. Natl. Acad. Sci. U.S.A. 84 (16), 5962-5966. Bulleit,R.F., Bennett,M.K., Molloy,S.S., Hurley,J.B. and Kennedy,M.B. (1988) Conserved and variable regions in the subunits of brain type II Ca2+/calmodulin-dependent protein kinase. Neuron 1 (1), 63-72. Hanley,R.M., Means,A.R., Ono,T., Kemp,B.E., Burgin,K.E., Waxham,N. and Kelly,P.T. (1987) Functional analysis of a complementary DNA for the 50-kilodalton subunit of calmodulin kinase II. Science 237 (4812), 293-297.
|
| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
|
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot using Rockland's Affinity Purified anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 μg of a mouse brain whole cell lysate (p/n W10-000-T004) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase II was similarly detected on lysates from rat brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye？800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature. IRDye？800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
|
|
|
|
Western blot using Rockland's Affinity Purified anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 μg of a mouse brain whole cell lysate (p/n W10-000-T004) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase II was similarly detected on lysates from rat brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye？800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature. IRDye？800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
|
|
|
| メーカー |
品番 |
包装 |
|
RKL
|
600-401-873
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
|
|
