| 別品名 |
Cyclic AMP-dependent transcription factor ATF-3 cAMP-dependent transcription factor ATF-3 Activating transcription factor 3
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| 抗原部位 |
a.a.113-130
|
| 種由来 |
Human
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| 標識物 |
Unlabeled
|
| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
|
| 交差種 |
Human
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| GENE ID |
467
|
| Accession No.(Gene/Protein) |
NP_001025458, P18847
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| Gene Symbol |
ATF3
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| 形状 |
滅菌済み液状品
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| 参考文献 |
[Pub Med ID]20056838
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Rockland's Affinity purified anti ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. Immunostaining using Rockland's anti HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDyeTM800 conjugated Gt a Rabbit IgG [H&L] (code 611 132 122) for 30 min at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Western blot of E.coli whole cell extract transfected with GST epitope tagged human ATF3. Rockland's Affinity purified anti-ATF3 detects a band ~48 kDa corresponding to recombinant human ATF3. Immunostaining using Rockland's anti-GST epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Rockland's Affinity purified anti ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. Immunostaining using Rockland's anti HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDyeTM800 conjugated Gt a Rabbit IgG [H&L] (code 611 132 122) for 30 min at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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| メーカー |
品番 |
包装 |
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RKL
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600-401-493
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100 UG
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※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
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