別品名 |
Cyclin B1, CCNB1, CCNB, G2/mitotic-specific cyclin-B1
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抗原部位 |
a.a.120-131
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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交差種 |
Human
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翻訳後修飾 |
リン酸化
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GENE ID |
891
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Accession No.(Gene/Protein) |
P14635
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Gene Symbol |
CCNB1
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形状 |
滅菌済み液状品
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参考文献 |
Davy,C.E., et al. (2005) Human papillomavirus type 16 E1 E4-induced G2 arrest is associated with cytoplasmic retention of active Cdk1/cyclin B1 complexes. J. Virol. 79 (7), 3998-4011. Innocente,S.A. and Lee,J.M. (2005) p53 is a NF-Y- and p21-independent, Sp1-dependent repressor of cyclin B1 transcription. FEBS Lett. 579 (5), 1001-1007. Torgler,R., Jakob,S., Ontsouka,E., Nachbur,U., Mueller,C., Green,D.R. and Brunner,T. (2004) Regulation of activation-induced Fas (CD95/Apo-1) ligand expression in T cells by the cyclin B1/Cdk1 complex. J. Biol. Chem. 279 (36), 37334-37342.
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot using Rockland's Affinity Purified anti-Cyclin B1 pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B1 (arrowheads) in various whole cell lysates.? Lane 1: Hela (cervical carcinoma) [p/n W09-000-364], Lane 2: H23 (lung carcinoma), Lane 3: Hep3b (Hepatocarcinoma), Lane 4: T98G (Glioblastoma), and Lane 5: Daudi (B cell lymphoblast) [W09-001-MQ2]. Each lane contains approximately 50 μg of lysates, separated by 12% SDS-PAGE using a 5% stack run at 100 volts until the dye front cleared the bottom of the gel.? Transfer occurred overnight at 4°C at 15 mAmps.? The membrane was blocked with 5% non-fat dry milk in TTBS (p/n B501-0500) for 1 h at room temperature followed by addition of a 1:100 dilution of the antibody allowed to react for 2h at room temperature.? After washes with TTBS a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-103-122) was added for 1 h at room temperature.? After additional washes the membrane was incubated with ECL mix 1:1 for ~3 min.? Excess detection solution was drained off and the membrane was exposed to Kodak film X-omat blue XB-1 for about 20 sec. Other detection systems will yield similar results.? Personnel Communication, Luca Cote, Temple U.
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Western blot using Rockland's Affinity Purified anti-Cyclin B1 pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B1 (arrowheads) in various whole cell lysates.? Lane 1: Hela (cervical carcinoma) [p/n W09-000-364], Lane 2: H23 (lung carcinoma), Lane 3: Hep3b (Hepatocarcinoma), Lane 4: T98G (Glioblastoma), and Lane 5: Daudi (B cell lymphoblast) [W09-001-MQ2]. Each lane contains approximately 50 μg of lysates, separated by 12% SDS-PAGE using a 5% stack run at 100 volts until the dye front cleared the bottom of the gel.? Transfer occurred overnight at 4°C at 15 mAmps.? The membrane was blocked with 5% non-fat dry milk in TTBS (p/n B501-0500) for 1 h at room temperature followed by addition of a 1:100 dilution of the antibody allowed to react for 2h at room temperature.? After washes with TTBS a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-103-122) was added for 1 h at room temperature.? After additional washes the membrane was incubated with ECL mix 1:1 for ~3 min.? Excess detection solution was drained off and the membrane was exposed to Kodak film X-omat blue XB-1 for about 20 sec. Other detection systems will yield similar results.? Personnel Communication, Luca Cote, Temple U.
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メーカー |
品番 |
包装 |
RKL
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600-401-464
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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