別品名 |
Serine threonine protein kinase Nek2 antibody, HSPK21 antibody, NEK 2 antibody, NEK2A antibody, NIMA (never in mitosis gene a) related kinase 2 antibody, NimA like protein kinase 1 antibody, NIMA related kinase 2 antibody
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抗原部位 |
a.a.287-299
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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交差種 |
Mouse
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GENE ID |
4751
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Accession No.(Gene/Protein) |
P51955
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Gene Symbol |
NEK2
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形状 |
滅菌済み液状品
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参考文献 |
Schultz,S.J., Fry,A.M., Sutterlin,C., Ried,T. and Nigg,E.A. (1994) Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans. Cell Growth Differ. 5 (6), 625-635. Schultz,S.J. and Nigg,E.A. (1993) Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans. Cell Growth Differ. 4 (10), 821-830.
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot analysis is shown using Rockland's Affinity Purified anti NEK2 antibody to detect endogenous protein present in an unstimulated mouse A 20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.
Possible model for NEK2 action.
Western Blot of Rabbit Anti-NEK2 Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (50ng). Lane 3: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (20ng). Lane 4: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (20ng). Lane 5: Jurkat cell lysate (10ug) (p/n W09-001-370). Primary Antibody: Anti-NEK2 at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 30mins at RT. Block: BlockOut Buffer (p/n MB-073). Expect/Observed MW: ~76kDa rec NEK2.
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Western blot analysis is shown using Rockland's Affinity Purified anti NEK2 antibody to detect endogenous protein present in an unstimulated mouse A 20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.
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メーカー |
品番 |
包装 |
RKL
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600-401-452
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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