| 別品名 |
GSK 3 alpha antibody, DKFZp686D0638 antibody, Glycogen synthase kinase 3 alpha antibody, GSK 3 alpha antibody, GSK 3A antibody, GSK3A antibody, GSK3alpha antibody
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| 抗原部位 |
a.a.16-25
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
|
| 交差種 |
Human
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| GENE ID |
2931
|
| Accession No.(Gene/Protein) |
P49840
|
| Gene Symbol |
GSK3A
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Gaster,M., et al. (2004) The primary defect in glycogen synthase activity is not based on increased glycogen synthase kinase-3alpha activity in diabetic myotubes. Biochem. Biophys. Res. Commun. 319 (4), 1235-1240. Barry,F.A., Graham,G.J., Fry,M.J. and Gibbins,J.M. (2003) Regulation of glycogen synthase kinase 3 in human platelets: a possible role in platelet function?. FEBS Lett. 553 (1-2), 173-178. Nikoulina,S.E., Ciaraldi,T.P., Mudaliar,S., Carter,L., Johnson,K. and Henry,R.R. (2002) Inhibition of glycogen synthase kinase 3 improves insulin action and glucose metabolism in human skeletal muscle. Diabetes 51 (7), 2190-2198. He,X., Saint-Jeannet,J.P., Woodgett,J.R., Varmus,H.E. and Dawid,I.B. (1995) Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos. Nature 374 (6523), 617-622.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot using Rockland's Affinity Purified anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A present in ~ 35 μg of HEK293 whole cell lysate (p/n W09-000-365). A 4-20% Tris Glycine gel was used for separation followed by blocking with 5% BLOTTO (p/n B501-0500) in PBS. Primary antibody was used at a 1:1,000 dilution in blocking buffer and reacted overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800？ conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye？800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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Western blot using Rockland's Affinity Purified anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A present in ~ 35 μg of HEK293 whole cell lysate (p/n W09-000-365). A 4-20% Tris Glycine gel was used for separation followed by blocking with 5% BLOTTO (p/n B501-0500) in PBS. Primary antibody was used at a 1:1,000 dilution in blocking buffer and reacted overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800？ conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye？800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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| メーカー |
品番 |
包装 |
|
RKL
|
600-401-444
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100 UG
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※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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