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Western blot using Rockland's Affinity Purified anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A in various human derived 293T cell extracts.？ Cells were serum starved for 24 h.？？ Lane 1: control, Lane 2: treated with IGF-1 (100 ng/ml) for 20 min, Lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min.？ Lane 1 shows some baseline GSK3A reactivity that is intensified upon stimulation (lane2) and diminished when an inhibitor is added (lane 3).？ Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1,000 dilution of anti-GSK3A antibody.？ Signal was detected using standard techniques.？ Personal communication, Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada
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Western blot using Rockland's Affinity Purified anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A in various human derived 293T cell extracts.？ Cells were serum starved for 24 h.？？ Lane 1: control, Lane 2: treated with IGF-1 (100 ng/ml) for 20 min, Lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min.？ Lane 1 shows some baseline GSK3A reactivity that is intensified upon stimulation (lane2) and diminished when an inhibitor is added (lane 3).？ Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1,000 dilution of anti-GSK3A antibody.？ Signal was detected using standard techniques.？ Personal communication, Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada
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| 別品名 |
rabbit anti-GSK3 alpha pS21 antibody, rabbit anti-GSK3α pS21 antibody, GSK 3 alpha pS21 antibody, Glycogen synthase kinase-3 alpha antibody, phosphorylated GSK-3 alpha antibody, GSK 3A antibody, GSK-3A antibody, GSK3alpha antibody, Serine/threonine-protein kinase GSK3A
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 抗原部位 |
a.a.16-25
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 翻訳後修飾 |
リン酸化
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| GENE ID |
2931
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| Accession No.(Gene/Protein) |
49574532, P49840
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| Gene Symbol |
GSK3A
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| 参考文献 |
Gaster,M., et al. (2004) The primary defect in glycogen synthase activity is not based on increased glycogen synthase kinase-3alpha activity in diabetic myotubes. Biochem. Biophys. Res. Commun. 319 (4), 1235-1240. Barry,F.A., Graham,G.J., Fry,M.J. and Gibbins,J.M. (2003) Regulation of glycogen synthase kinase 3 in human platelets: a possible role in platelet function?. FEBS Lett. 553 (1-2), 173-178. Nikoulina,S.E., Ciaraldi,T.P., Mudaliar,S., Carter,L., Johnson,K. and Henry,R.R. (2002) Inhibition of glycogen synthase kinase 3 improves insulin action and glucose metabolism in human skeletal muscle. Diabetes 51 (7), 2190-2198.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-429
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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