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Western Blot of Rabbit Anti-AKT2 antibody. Load: 20mg/lane. Lane 1: HEK293 lysate [Control]. Lane 2: HEK293 lysate + 15 min 4OHT (tamoxifen). Lane 3: HEK293 lysate + 30 min 4OHT (tamoxifen). Lane 4: HEK293 lysate pretreated for 15min with 25uM LY294002. Lane 5: HEK293 lysate + 15 min 4OHT (tamoxifen) lysate pretreated for 15min with 25uM LY294002. Lane 6: HEK293 lysate + 30 min 4OHT (tamoxifen) lysate pretreated for 15min with 25uM LY294002. Primary antibody: Anti-AKT2 Antibody at 1:1000 for 1h at room temperature. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Observed size: Endogenous Akt2 ~60kDa, myristoylated construct ~110kDa. Other band(s): This treatment has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2.
Western Blot of Rabbit anti-AKT2 antibody. Lane 1: HEK293 lysate [control]. Lane 2: HEK293 lysate pretreated for 15min with 25uM LY294002. Load: 20mg/lane. Primary antibody: AKT2 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed: phosphorylated (top arrow) and the non-phosphorylated (bottom arrow) forms of endogenous Akt2. Other band(s): nonspecific.
Western Blot of Rabbit anti-AKT2 antibody. Lane 1: mouse skeletal muscle tissue lysates. Lane 2: mouse fat tissue lysates. Lane 3: mouse liver tissue lysates. Load: 35 μg per lane. Primary antibody: AKT2 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
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Western Blot of Rabbit Anti-AKT2 antibody. Load: 20mg/lane. Lane 1: HEK293 lysate [Control]. Lane 2: HEK293 lysate + 15 min 4OHT (tamoxifen). Lane 3: HEK293 lysate + 30 min 4OHT (tamoxifen). Lane 4: HEK293 lysate pretreated for 15min with 25uM LY294002. Lane 5: HEK293 lysate + 15 min 4OHT (tamoxifen) lysate pretreated for 15min with 25uM LY294002. Lane 6: HEK293 lysate + 30 min 4OHT (tamoxifen) lysate pretreated for 15min with 25uM LY294002. Primary antibody: Anti-AKT2 Antibody at 1:1000 for 1h at room temperature. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Observed size: Endogenous Akt2 ~60kDa, myristoylated construct ~110kDa. Other band(s): This treatment has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2.
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| 別品名 |
rabbit anti-AKT2 antibody, AKT 2, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta
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| 交差種 |
Human
Mouse
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 抗原部位 |
a.a.455-468
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
164731
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| Accession No.(Gene/Protein) |
NP_001317440.1, P31751
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| Gene Symbol |
AKT2
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| 参考文献 |
Altomare, D. A., Kozak, C. A., Sonoda, G. and Testa, J. R. (1996) Chromosome mapping of the mouse Akt2 gene and Akt2 pseudogene. Cytogenet. Cell Genet. 74: 248-251. PubMed ID : 8976376 Cheng, J. Q. wt al. (1992) AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas. Proc. Nat. Acad. Sci. 89: 9267-9271. PubMed ID : 1409633 Cheng, J. Q. et al. (1996) Amplification of AKT2 in human pancreatic cancer cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA. Proc. Nat. Acad. Sci. 93: 3636-3641. PubMed ID : 8622988 Subramanyam M, Takahashi N, Hasegawa Y, et al. Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase. J. Biol. Chem. 2010 Feb 26;285(9):6109-6117
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-425
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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