| 別品名 |
E3 ubiquitin-protein ligase Mdm2 EC=6.3.2. p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein
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| 抗原部位 |
a.a.177-198
|
| 種由来 |
Mouse
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| 標識物 |
Unlabeled
|
| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
Mouse
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| 翻訳後修飾 |
リン酸化
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| GENE ID |
17246
|
| Accession No.(Gene/Protein) |
P23804
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| Gene Symbol |
MDM2
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Pospisilova S, Siligan C, Ban J, Jug G, Kovar H. (2004) Constitutive and DNA Damage Inducible Activation of pig3 and MDM2 Genes by Tumor-Derived p53 Mutant C277Y. Mol Cancer Res. 2(5):296-304. Aslanian A, Iaquinta PJ, Verona R, Lees JA. (2004) Repression of the Arf tumor suppressor by E2F3 is required for normal cell cycle kinetics. Genes Dev. 2004 Jun 2 [Epub ahead of print]. Stros M, Muselikova-Polanska E, Pospisilova S, Strauss F. (2004) High-Affinity Binding of Tumor-Suppressor Protein p53 and HMGB1 to Hemicatenated DNA Loops. Biochemistry. 43(22):7215-7225.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Affinity Purified Anti-MDM2 pS185 (Rabbit) is shown to detect a 102 kDa band？ (arrow) corresponding to phosphorylated mouse MDM2 present in a 293T whole cell lysate.？？ Cells were serum-starved for 24 hours prior to harvest.？ Approximately 20 μg of lysate was loaded per lane for SDS-PAGE.？ Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest.？ Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
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Affinity Purified Anti-MDM2 pS185 (Rabbit) is shown to detect a 102 kDa band？ (arrow) corresponding to phosphorylated mouse MDM2 present in a 293T whole cell lysate.？？ Cells were serum-starved for 24 hours prior to harvest.？ Approximately 20 μg of lysate was loaded per lane for SDS-PAGE.？ Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest.？ Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
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| メーカー |
品番 |
包装 |
|
RKL
|
600-401-423
|
100 UG
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※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
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