別品名 |
FKBP12 rapamycin complex associated protein antibody, FLJ44809 antibody, FRAP antibody
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抗原部位 |
a.a.2440-2457
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry
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免疫動物 |
Rabbit
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交差種 |
Human
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翻訳後修飾 |
リン酸化
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GENE ID |
2475
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Accession No.(Gene/Protein) |
P42345
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Gene Symbol |
FRAP1
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]26105008, 29906337
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Rockland's affinity purified anti mTOR pS 2448 antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi human, multi brain and multi cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney (40X). Tissue was formalin fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Affinity Purified Anti-mTOR pS 2448 (Rabbit) is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
Metabolic and autocrine regulation of the mTOR pathway by Beta-cells.
ELISA Results of Rabbit Anti-mTOR pS2448 Antibody tested against BSA-conjugated non-phospho [purple line] and phospho [blue line] forms of immunizing peptide. Each well was coated in duplicate with either 0.1ug of conjugate. The working dilution is 1:37,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugate stabilizer (p/n MB-067), Goat Anti-Rabbit HRP conjugated (p/n 611-103-122) and TMB substrate (p/n TMBE-1000).
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Rockland's affinity purified anti mTOR pS 2448 antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi human, multi brain and multi cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney (40X). Tissue was formalin fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
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メーカー |
品番 |
包装 |
RKL
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600-401-422
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100 UG
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※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
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保存温度 |
-20℃
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