Anti 6X His Epitope Tag  

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot.？ Polyclonal rabbit

Western blot of Rabbit Anti-6xHIS Epitope Tag antibody. Lane: NFR1-HIS recombinant protein. Load: 50 ng per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4°C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.

(C and D) The C-terminally 8×Myc-tagged PhoR protein immunoprecipitates PhoU-His. (C) Crude extracts prepared from？Salmonella？strains with the？phoR-8×Myc gene expressing PhoU-His, PhoUR184A-His, PhoUR184G-His, or the empty vector (pBAD33) were detected with anti-Myc (top) and anti-His (bottom) antibodies. (D) Eluted fractions prepared from strains listed above were detected with anti-Myc (top) and anti-His (bottom) antibodies after immunoprecipitation with anti-Myc antibody-coated beads. Fig 4. PMID:？35638741

CHD4 modulates vascular Wnt signaling at two levels. (A) Chromatin immunoprecipitation (ChIP) assays were performed using antibodies against CHD4 or a polyhistidine epitope tag as a negative control. DNA was isolated and amplified by qPCR to determine whether CHD4 bound the promoter region of？Tcf7？or？Lef1.？(B) Chromatin harvested from nonspecific (NS) or CHD4 siRNA-transfected C166 yolk sac endothelial cells was immunoprecipitated with an antibody against histone H3 or a negative-control antibody (p/n 600-401-382). DNA was isolated and amplified by qPCR to examine the relative nucleosome density at the？Tcf7？promoter. (C) Chromatin immunoprecipitation assays were carried out using antibodies against CHD4 or IgG (negative control) to determine whether CHD4 was associated with the promoter region of the Wnt target genes？Pitx2,？Myc,？Ccnd1, and？Plaur. (A to C) A region greater than 5 kb upstream of the？Fzd5？transcription start site (Fzd5UP) was used as a negative control. Data from three independent experiments were combined and are presented as fold enrichment over the level with the negative-control antibody. Significant differences were calculated using a two-tailed Student？t？test (*,？P？< 0.05). Fig 5. PMID:？22290435

Ssa1 S151 phosphorylation is mediated by Cdk1 and regulated by the TOR pathway. (A) Δssa1-4？yeast cells expressing GFP-Flag-Ssa1 (WT) or GFP-Flag-Ssa1 S151A (S151A) as well as galactose-inducible HA-His-tagged Cdk1 (Gal-yCDC28-HA-His) were grown in glucose (Glu) or galactose (Gal). Ssa1 was isolated by immunoprecipitation and analyzed by Western blotting with anti-phospho-Hsp70(S151/153) (pSSA1), anti-His, and anti-Flag antibodies. Fig 3. PMID:？32205407