Anti NFkB p65  

Rockland Immunochemical's Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in (A) control DU145 cells and (B) TNF stimulated DU145 cells.？ Although DU145 show relatively high basal levels of nuclear p65 staining, significant enhancement of nuclear staining is seen in panel B as evidence of translocation and availability of the NLS to be bound by the antibody.？ Cultured cells shown above were formalin-fixed.？ Tissue staining (not shown) were formalin-fixed, paraffin embedded followed by citrate retrieval.？ Blocking and hybridization included 5% NGS.

Anti-p65 NLS Antibody and Anti NFkB monoclonal antibody ？ Immunocytochemistry. Tissue: Human Fibroblasts. Top two: Before activation. Bottom two: After activation with poly IC. Left: 600-401-271: anti-p65 NLS specific lot 18372. Right: 200-301-065: Monoclonal anti-NFkB p65 antibody C-Term. The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.

Immunofluorescence Microscopy of Anti-p65 NLS Antibody. Cells: TNF stimulated DU145 cells - fixed. Antibody: Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65. Image shown is at a 1:400 magnification.

ELISA results of purified Rabbit anti-NFkB p65 NLS Specific Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1μg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-103-122) and TMB ELISA Peroxidase Substrate (p/n TMBE-1000).