別品名 |
NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immuno Fluorescence
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免疫動物 |
Rabbit
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交差種 |
Human
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GENE ID |
5970
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Accession No.(Gene/Protein) |
Q04206
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Gene Symbol |
NFKB1
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]16644679, 25108526, 29637625, 30244868, 34768848
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunofluorescence Microscopy of Anti p65 NLS Antibody. Cells: TNF stimulated DU145 cells fixed. Antibody: Rabbit anti p65 NLS was used at a 1:200 dilution to detect p65. Image shown is at a 1:400 magnification.
Rockland Immunochemical's Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in (A) control DU145 cells and (B) TNF stimulated DU145 cells.? Although DU145 show relatively high basal levels of nuclear p65 staining, significant enhancement of nuclear staining is seen in panel B as evidence of translocation and availability of the NLS to be bound by the antibody.? Cultured cells shown above were formalin-fixed.? Tissue staining (not shown) were formalin-fixed, paraffin embedded followed by citrate retrieval.? Blocking and hybridization included 5% NGS.
ELISA results of purified Rabbit anti-NFkB p65 NLS Specific Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-103-122) and TMB ELISA Peroxidase Substrate (p/n TMBE-1000).
Anti-p65 NLS Antibody and Anti NFkB monoclonal antibody ? Immunocytochemistry. Tissue: Human Fibroblasts. Top two: Before activation. Bottom two: After activation with poly IC. Left: 600-401-271: anti-p65 NLS specific lot 18372. Right: 200-301-065: Monoclonal anti-NFkB p65 antibody C-Term. The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.
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Immunofluorescence Microscopy of Anti p65 NLS Antibody. Cells: TNF stimulated DU145 cells fixed. Antibody: Rabbit anti p65 NLS was used at a 1:200 dilution to detect p65. Image shown is at a 1:400 magnification.
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メーカー |
品番 |
包装 |
RKL
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600-401-271
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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