別品名 |
I kappa B kinase epsilon antibody, IkBKE antibody, IKBKE protein antibody, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody, Inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, IKKE, IKKI, KIAA0151
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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交差種 |
Human
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翻訳後修飾 |
リン酸化
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GENE ID |
9641
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Accession No.(Gene/Protein) |
Q14164, Q9R0T8
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Gene Symbol |
IKKε
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]31455651, 31434731, 17328045
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
IFI44 decreases the kinase activity of IKKβ and IKKε. Human 293T cells were silenced for IFI44, or for FKBP5, and were transfected with plasmids expressing His-IKKε (A) or MYC-IKKβ (B), together with IFI44-HA, and FKBP5-FLAG expression plasmids. At 24 hpt, IKKε (A) and IKKβ (B) complexes were purified with anti-His and anti-MYC antibodies, respectively, and these complexes were assayed in kinase assays using IRF-3 (for the IKKε complexes shown in panel A) and IkBα (for the IKKβ complexes shown in panel B) as substrates. The levels of phosphorylated and unphosphorylated forms of IRF-3 (panel A, bottom blot) and IkBα (panel B, third and fourth blots) were analyzed by Western blotting using specific antibodies. Levels of IKKε were analyzed using an anti-His-specific antibody (A, first blot) and anti-pIKKε (A, second blot), and levels of IKKβ were analyzed using an anti-MYC-specific antibody (B, first blot) and anti-pIKKβ (B, second blot). Western blots were quantified by densitometry using ImageJ software (v1.46). Protein expression levels in cells expressing IKKε (A) and IKKβ (B) alone were assigned a value of 100% for comparisons with the levels of expression in cells expressing the different combinations of IKKε/IFI44/FKBP5 (A) or IKKβ/IFI44/FKBP5 (B) (numbers are indicated below each plot). pIRF-3 and IRF-3 levels (observed in the same bottom blot in panel A) and pIkBα and IkBα (third and bottom blot in panel B) are represented with numbers below each blot. Levels of pIRF-3 and pIkBα normalized to the levels of IKKε and IKKβ are represented in the bottom graphs in panels A and B, respectively. Molecular weight markers are indicated (in kilodaltons) on the right. Figure provided by CiteAb. Source: MBio, PMID: 31455651.
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IFI44 decreases the kinase activity of IKKβ and IKKε. Human 293T cells were silenced for IFI44, or for FKBP5, and were transfected with plasmids expressing His-IKKε (A) or MYC-IKKβ (B), together with IFI44-HA, and FKBP5-FLAG expression plasmids. At 24 hpt, IKKε (A) and IKKβ (B) complexes were purified with anti-His and anti-MYC antibodies, respectively, and these complexes were assayed in kinase assays using IRF-3 (for the IKKε complexes shown in panel A) and IkBα (for the IKKβ complexes shown in panel B) as substrates. The levels of phosphorylated and unphosphorylated forms of IRF-3 (panel A, bottom blot) and IkBα (panel B, third and fourth blots) were analyzed by Western blotting using specific antibodies. Levels of IKKε were analyzed using an anti-His-specific antibody (A, first blot) and anti-pIKKε (A, second blot), and levels of IKKβ were analyzed using an anti-MYC-specific antibody (B, first blot) and anti-pIKKβ (B, second blot). Western blots were quantified by densitometry using ImageJ software (v1.46). Protein expression levels in cells expressing IKKε (A) and IKKβ (B) alone were assigned a value of 100% for comparisons with the levels of expression in cells expressing the different combinations of IKKε/IFI44/FKBP5 (A) or IKKβ/IFI44/FKBP5 (B) (numbers are indicated below each plot). pIRF-3 and IRF-3 levels (observed in the same bottom blot in panel A) and pIkBα and IkBα (third and bottom blot in panel B) are represented with numbers below each blot. Levels of pIRF-3 and pIkBα normalized to the levels of IKKε and IKKβ are represented in the bottom graphs in panels A and B, respectively. Molecular weight markers are indicated (in kilodaltons) on the right. Figure provided by CiteAb. Source: MBio, PMID: 31455651.
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メーカー |
品番 |
包装 |
RKL
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600-401-267
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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