Anti GFP  

Immuno-microscopy of Rabbit anti-GFP antibody. Monocyte derived dendritic cells and dermal macrophages were challenged and directly visualized with eGFP labeled Dengue virus to localize sequestration of virus particles in the different cells (upper). The location of the GFP was confirmed by TEM (lower magnified view) using Rockland rabbit anti GFP Primary antibody (1:200) and a gold labeled secondary antibody. As referenced in: Kwan W-H, Navarro-Sanchez E, Dumortier H, Decossas M, Vachon H, et al. (2008) Dermal-Type Macrophages Expressing CD209/DC-SIGN Show Inherent Resistance to Dengue Virus Growth. PLoS Negl Trop Dis 2(10): e311. doi:10.1371/journal.pntd.0000311

Western Blot of Rabbit anti-GFP antibody. Lane 1: Wild type GFP (0.1 μg) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 μg per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800^TM Goat-a-Rabbit IgG [H&L] MX10 (611-132-122) at 1:10,000 for 45 min at RT. Block: 5% BLOTTO in PBS overnight at 4°C. Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.

Western Blot of Rabbit anti-GFP antibody. Lane 1: 293FT cells transfected with CDK4 dominant negative (C-). Lane 2: 293FT cells poitive control (C+). Load: 25 μg per lane. Primary antibody: GFP antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800^TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 27 kDa for GFP.

Binding of MTBP to chromatin requires TRESLIN in G1 but not in S.？A？Protein levels of MTBP-mClover and TRESLIN-mClover in HCT116 cells were measured via flow cytometry using an anti-GFP antibody, alongside DNA content. This analysis was conducted 24？h after siRNA transfection, representing a single experimental replicate.？B？Quantification of the data in panel？A？involved background-subtracting individual cell anti-GFP immunofluorescence signals, averaging signals for cells in 2？N, early S, or 4？N DNA content gates, and normalizing values from siTRESLIN or siMTBP transfected cells to siCNTRL. The means of these normalized values across three replicates are shown with error bars indicating 95% confidence intervals. Asterisks indicate significance levels from？t-tests comparing each sample to its respective siCNTRL.？C？D？replicate (A) and (B), respectively, with the exception that soluble protein was pre-extracted before fixation. Consequently, immunofluorescence signals represent insoluble TRESLIN-mClover or MTBP-mClover protein. Fig 4. PMID:？40624716

Western Blot of Rabbit anti-GFP antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: CHO/K1 Lysate (p/n W07-000-357). Lane 4: MDA-MB-231 (p/n W09-001-GK6). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: Jurkat Lysate (p/n W09-001-370). Lane 7: NIH/3T3 Lysate (p/n W10-000-358). Lane 8: E-coli HCP Control (p/n 000-001-J08). Lane 9: FLAG Positive Control Lysate (p/n W00-001-383). Lane 10: Red Fluorescent Protein (p/n 000-001-379). Lane 11: Green Fluorescent Protein (p/n 000-001-215). Lane 12: Glutathione-S-Transferase Protein (p/n 000-001-200). Lane 13: Maltose Binding Protein (p/n 000-001-385). Load: 10 μg of lysate or 50ng of purified protein per lane. Primary antibody: GFP antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 30 kDa for GFP.

Western Blot of Rabbit Anti-GFP Antibody. Lane 1: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: GFP (p/n 000-001-215) / HeLa Lysate (p/n W09-000-364) [0.1μg/10.0μg]. Lane 3: GFP (p/n 000-001-215) / HeLa Lysate (p/n W09-000-364) [0.05μg/10.0μg]. Lane 4: GFP (p/n 000-001-215) / HeLa Lysate (p/n W09-000-364) [0.03μg/10.0μg]. Lane 5: HeLa Lysate (p/n W09-000-364) [10.0μg]. Primary Antibody: Rabbit Anti-GFP Antibody at 1.0μg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG MX 10 Peroxidase (p/n 611-103-122) at 1:70,000 for 30mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 60 mins at RT. Expect: ~27kDa.

ELISA results of purified Rabbit Anti-GFP Antibody. Each well was coated in 10 μg of antigen GFP [Red Line], human IgG [Green Line], Mouse IgG [Blue Line], and Rat IgG [Purple Line]. The starting dilution of antibody was 5 μg/mL and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:67,700. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 1% Fish Gel, TMB Substrate (p/n TMB-1000), and Goat Anti-Rabbit IgG Antibody HRP (p/n 611-103-122).

B？Insoluble flow cytometry showing level of chromatin bound MCM7 and TRESLIN-mClover or MTBP-mClover in tagged (TRESLIN or MTBP) or untagged (HCT-116) cells. Anti-MCM7, anti-GFP, or anti-IgG immunofluorescence signal vs DNA content is plotted to show the level of each protein across the cell cycle. Treatment with doxycycline for 16？h was sufficient to reduce levels of chromatin bound MCM7 in 2？N cells. TRESLIN binding was unaffected while MTBP binding was partially reduced. Fig 5. PMID:？40624716

Chromosomal ALFA-tagged MmpL3 and RpoC. (A) Immunoblots of lysates from MC2155 (WT), MGM7103 (encoding RpoC-ALFA), and MGM7059 (encoding MmpL3-ALFA). The top blot is probed using NBALFA and anti-VHH-HRP. The bottom blot is stripped and probed with anti-SigA and anti-rabbit Ig-horseradish peroxidase (HRP) as a loading control. (B) Immunoblots of lysates from MC2155, MGM6970 (expressing GFP), MGM7087 (expressing GFP-NBALFA from the PftsZ1？promoter), MGM7089 (expressing GFP-NBALFA from the Phsp60？promoter), MGM7047 (expressing TetON GFP-NBALFA, no ATc), and MGM7047 (encoding TetON GFP-NBALFA, +ATc-50 ng/mL). Top blot probed with anti-GFP and anti-rabbit Ig-HRP. Bottom blot stripped and probed with anti-SigA and anti-rabbit Ig-HRP. Fig 2. PMID:？40576343

(A-D”’) Pairs of SR-SIM images showing two sides of the same cells that express Bcd1-GFP and are either otherwise wild-type (A-B”’) or？hpo1-3？(C-D”’). The cells were labeled with the anti-GFP antibodies (red), 20H5 anti-centrin antibody (green), and DAPI (blue) after a period of growth for 4 hours at 39°C. SF5. PMID:？40455876