Anti Fibronectin  

Immunofluorescence of Anti-Fibronectin Antibody. Representative images of isolated EC cultured on various MA-coated surfaces after 24 h exposure to 0.5 dyn/cm2 immunofluorescence-labelled for Fibronectin. A strong rearrangement of the initial Fibronectin layer into coarse fibrils (under venous shear stress) (Fig. 3I) with severe displacements of Fibronectin occurred on PPMA-p. Only slight Fibronectin reorganization into fine fibrils (PPMA-c Fig. 3J) or no Fibronectin reorganization at all (POMA-p and POMA-c Fig. 3K and L) were observed as expected from the higher Fibronectin anchorage strength to these latter substrates in comparison to PPMA-p. These findings are in line with earlier results at static cell culture conditions of isolated EC [4e6] showing the dependence of adhesion and stress fibre patterns on the matrix anchorage to the polymer surface, which were now attenuated by the application of shear stress.Scale bar: 10 mm. Fig. 3. PMID: 22154622.