Anti Collagen Type VI  

Rockland anti collagen VI antibody (600-401-108 Lot 26009, 1:400 45 min RT) showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA

Dot Blot results using Rabbit Anti-Collagen VI Antibody. Sample: A) Human Collagen VI (p/n 009-001-108), B) Bovine/Human Collagen VI (p/n 001-001-108).Load: 1) 1000ng, 2) 333.33ng, 3) 111.11ng, 4) 37.03ng, 5) 12.35ng.Primary Antibody: Rabbit Anti-Collagen VI antibody (p/n 600-401-108) at 5μg/mL at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated (p/n 611-1302) 1:40,000 at RT for 30mins. Blocking: BlockOutR Universal Blocking Buffer (p/n MB-073) at RT for 30mins.

Western blot results using Rabbit Anti-Collagen Type VI Antibody. Lane: 1) Human Collagen Type VI (p/n 009-001-108) reduced and boiled, 3) Human Collagen Type VI reduced and not boiled,5) Opal prestain molecular weight marker (p/n MB-210-0500), 7) Human Collagen Type VI not reduced and boiled, 9) Human Collagen Type VI not reduced and not boiled. Lanes 2, 4, 6, 8 blank. Load: 10μg. Primary Antibody: Rabbit Anti-Collagen Type VI Antibody (p/n 600-401-108) 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG HRP conjugated (p/n 611-1302) 1:40,000 at RT for 30mins. Blocking: BlockOutR Universal Blocking Buffer (p/n MB-073) at RT for 1hr.