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Rockland's Affinity Purified anti-Collagen I antibody was used at a 1:100 dilution to detect distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. The antibody was reacted with antibody for 4 hours at room temperature followed by the addition of secondary antibody and substrate reaction. Tissue was formalin-fixed and paraffin embedded. No antigen retrieval was performed.
Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1 (p/n 009-001-103). Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight？ 649 rabbit secondary antibody (p/n 611-743-127) at 1:20,000 for 30 min at RT. Block: (p/n MB-070( for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.
Immunohistochemistry of Rabbit Anti-collagen type I antibody. Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:1250 for 4°C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type I is intra and extracellular. Staining: 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.
Immunohistochemistry of Collagen I antibody. Tissue: human lung. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Immunohistochemistry results of Rabbit Anti-Collagen Type I Antibody. Tissue: human stomach mucosa (TMA). Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type I (p/n 600-401-103-0.1) at 1:15 for 1 hr at 37 °C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec.Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: basement membranes and blood vessels. Independently Validated by？antibodies-online GmbH (p/n ABIN7565871/ ABIN5596819/ ABIN5596820) by？MS Validated Antibodies.
Immunohistochemistry results of Rabbit Anti-Collagen Type I Antibody. Tissue: smooth muscle cells of human stomach wall. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type I (p/n 600-401-103-0.1) at 1:15 for 1 hr at 37 °C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec.Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: smooth muscle cells surrounded by collagen fibers. Independently Validated by？antibodies-online GmbH (p/n ABIN7565871/ ABIN5596819/ ABIN5596820) by？MS Validated Antibodies.
Immunohistochemistry results of Rabbit Anti-Collagen Type I Antibody. Tissue: human tubuli and blood vessels. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type I (p/n 600-401-103-0.1) at 1:15 for 1 hr at 37 °C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec.Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: Intense collagen I staining of fibres surrounding tubuli and around blood vessels. Independently Validated by？antibodies-online GmbH (p/n ABIN7565871/ ABIN5596819/ ABIN5596820) by？MS Validated Antibodies.
Immunohistochemistry results of Rabbit Anti-Collagen Type I Antibody. Tissue: human stroma of a colorectal adenocarcinoma. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type I (p/n 600-401-103-0.1) at 1:15 for 1 hr at 37 °C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec.Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: Cancer cells are collagen I negative. Independently Validated by？antibodies-online GmbH (p/n ABIN7565871/ ABIN5596819/ ABIN5596820) by？MS Validated Antibodies.
Immunohistochemistry using Rabbit Anti-Collagen Type I Antibody. Tissue: Human Skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: HEIR pH 6.0 buffer for 20 mins. Primary antibody: Anti-Collagen Type I Antibody (lot 52110) at 1:500 for 30 mins. Secondary antibody: Anti-Rabbit Poly-HRP IgG for 8 mins. Staining: DAB kit. Localization: Peri-follicular and dermal collagen. Size: W 164.6μm x L 206.2μm.
Immunohistochemistry using Rabbit Anti-Collagen Type I Antibody. Tissue: Human Skin. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-Collagen Type I Antibody (lot 52110) at 1:500 for 30 mins. Secondary antibody: Anti-Rabbit Poly-HRP IgG for 8 mins. Antigen retrieval: HEIR pH 6.0 buffer for 20 mins. Staining: DAB kit. Localization: Vessel and dermal Collagen. Size: W 193.0μm x L 202.7μm.
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Rockland's Affinity Purified anti-Collagen I antibody was used at a 1:100 dilution to detect distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. The antibody was reacted with antibody for 4 hours at room temperature followed by the addition of secondary antibody and substrate reaction. Tissue was formalin-fixed and paraffin embedded. No antigen retrieval was performed.
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| 別品名 |
rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1, Collagen type I alpha 2 antibody
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| 由来詳細 |
[Species]Human/Bovine
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| 交差種 |
Human
Mouse
Rat
Bovine
Porcine
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| 適用 |
Western Blot
Immunohistochemistry
Dot Blot
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
1277
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| Accession No.(Gene/Protein) |
NP_000079.2, P02452
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| Gene Symbol |
COL1A1/A2
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| 参考文献 |
[Pub Med ID]39980135
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-103-0.5
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0.5 MG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃
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