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Western Blot of human serum albumin. Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum. Lane 7: 1:10 of human serum albumin (10mg/mL). Lane 8: 1:40 of human serum albumin (10mg/mL). Lane 9: molecular weight. Right Blot: Secondary only incubated for control. Primary antibody: human serum albumin at 1:400 for overnight at 4°C. Secondary antibody: Biotin conjugated rabbit anti human serum albumin with 10 mg/ml BSA added to stock was diluted 1:5000 in PBST for 2hrs at RT. DyLight 649 conjugated Streptavidin 1:50000 in PBST 1 hr RT was used as a secondary reagent. Block: 1.5 % rabbit serum overnight at 4°C.
Dot Blot of Rabbit IgG protein. Antigen: control Rabbit IgG protein. Load: 250ng starting total load in a serial 1:1 dilution. Primary antibody: Rabbit IgG protein. Secondary antibody: Rabbit IgG Antibody Peroxidase Conjugated and Rabbit IgG Antibody DyLightTM 649 Conjugated secondary antibodies at 1:20,000 for 45 min at RT. Block: MB-070 buffer overnight at 4°C. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager.
PHH distribution within composite silk-collagen I scaffolds of small or large pore sizes. Silk scaffolds containing collagen I were fabricated with (A) small pores (73 ± 25 μm) or (B) large pores (235 ± 84 μm) and then PHHs were seeded into the scaffolds per the protocols described in methods. After 3 weeks, the cell-laden scaffolds were fixed, sectioned, and stained for intracellular albumin. Shown in both panels is a single section and magnified images from three different regions within the scaffold to show the distribution of PHH attachment. The scale bars in magnified images are 250 μm. Fig 2. PMID:？35515172
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Western Blot of human serum albumin. Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum. Lane 7: 1:10 of human serum albumin (10mg/mL). Lane 8: 1:40 of human serum albumin (10mg/mL). Lane 9: molecular weight. Right Blot: Secondary only incubated for control. Primary antibody: human serum albumin at 1:400 for overnight at 4°C. Secondary antibody: Biotin conjugated rabbit anti human serum albumin with 10 mg/ml BSA added to stock was diluted 1:5000 in PBST for 2hrs at RT. DyLight 649 conjugated Streptavidin 1:50000 in PBST 1 hr RT was used as a secondary reagent. Block: 1.5 % rabbit serum overnight at 4°C.
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| 別品名 |
rabbit anti-human serum albumin antibody, rabbit anti-HSA antibody, Analbuminemia antibody, rabbit anti-serum albumin antibody, rabbit anti-albumin antibody
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Dot Blot
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
213
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| Accession No.(Gene/Protein) |
NP_000468.1, P02768
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| Gene Symbol |
ALB
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| 参考文献 |
[[Cosmo Reference Collection]][Pub Med ID]35515172
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-033
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1 MG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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