|
※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Goat anti-GST antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: CHO/K1 Lysate (p/n W07-000-357). Lane 4: MDA-MB-231 (p/n W09-001-GK6). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: Jurkat Lysate (p/n W09-001-370). Lane 7: NIH/3T3 Lysate (p/n W10-000-358). Lane 8: E-coli HCP Control (p/n 000-001-J08). Lane 9: FLAG Positive Control Lysate (p/n W00-001-383). Lane 10: Red Fluorescent Protein (p/n 000-001-379). Lane 11: Green Fluorescent Protein (p/n 000-001-215). Lane 12: Glutathione-S-Transferase Protein (p/n 000-001-200). Lane 13: Maltose Binding Protein (p/n 000-001-385). Load: 10 μg of lysate or 50ng of purified protein per lane. Primary antibody: GST antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody (p/n 605-4302) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 26 kDa for GST.
Western Blot of Goat Anti-GST Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: rec GST [0.1μg]/ HeLa Whole Cell Lysate [10μg]. Lane 3: rec GST [0.05μg]/ HeLa Whole Cell Lysate [10μg]. Lane 4: rec GST [0.02μg]/ HeLa Whole Cell Lysate [10μg]. Lane 3: HeLa Whole Cell Lysate (p/n W09-000-364) [10μg]. Primary Antibody: Goat anti-GST antibody (p/n 600-101-200) at 1.0μg/mL for overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Goat IgG (H&L) Antibody Peroxidase Conjugated (p/n 605-4302) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) at RT for 30 min. Predicted/Observed size: ~26kDa.
Immunoprecipitation of anti-GST antibody. Lane 1: T98G cells incubated with GST-Pin1. Lane 2: T98G cells incubated with GST-CDK4/cyclinD1. Lane 3: T98G cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 μg per lane. Primary antibody: anti-GST 1:400 for overnight at 4°C. Secondary antibody: IRDye800TM secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
ELISA results of purified Goat Anti-GST Antibody tested against GST. Each well was coated in 1.0 μg of antigen. The starting dilution of antibody was 5 μg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer (p/n MB-060-1000), Substrate (p/n TMB-8000), and Donkey Anti-Goat IgG Antibody HRP (p/n 605-703-125) at 1:15,000.
(D) Pull-down assay. GST (？26 kDa) or GST-Mig1 (？81.5 kDa) were purified (Input, anti-GST) and bound to glutathione-sepharose beads (Pull Down, anti-GST). Beads were incubated with purified MBP-Opy2CT？(Input, anti-MBP, ？69.4 kDa). Coprecipitated MBP-Opy2CT？is shown (Pull Down, anti-MBP). Fig 3. PMID:？22904036
Recombinant proteins were blotted onto nitrocellulose membranes and reacted with patient serum. Arrows indicate positive phage clones. Positive clones were re-cloned for 2 additional times to obtain monoclonality？A.？GST protein (lane 1) and affinity-purified GST-tagged PDCD11 (lane 2) were separated on 11% SDS-polyacrylamide gels and stained with Coomassie Blue？B., or western blotted using anti-GST antibody？C.,？or the autologous sera of patients with aCI？D., TIA？E., and HD？F.？Asterisks indicate partially degraded proteins. Molecular weights are shown in the left. Fig 1.PMID:？29507658
F？I, pulldown experiments showing that ALIX and TSG101 bind to GST-MA expressed in？E. coli. The relative affinity for MA compared with p6 is higher for ALIX. GST-tagged Gag fragments (MA (aa 1？132), CA (aa 133？363), and p6 (aa 448？500)) were bound to GSH-Sepharose and incubated with extract of HEK293 cells expressing epitope-tagged ALIX or TSG101 from a CMV promoter vector. Bead-bound proteins were analyzed by immunoblotting with the indicated antibodies. α-Actin served as control, showing specific ESCRT protein binding. Fig 4. PMID:？32994219
G？K, pulldown experiments showing that genomically epitope-tagged Bro1 and Vps23 bind to GST-MA expressed in？E. coli？and that Bro1 additionally binds to GST-p6 dependent on the ALIX-binding motif. GST-tagged Gag fragments (MA (aa 1？132), CA (aa 133？363), and p6 (aa 448？500)) were bound to GSH-Sepharose and incubated with yeast extract. Bead-bound proteins were analyzed by immunoblotting with the indicated antibodies. PGK served as control, showing specific ESCRT protein binding.？G？and？J, binding buffer contained 150 mm？NaCl.？H,？I, and？K, binding buffer contained 400 mm？NaCl. Fig 3. PMID:？32994219
|
|
|
|
Western Blot of Goat anti-GST antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: CHO/K1 Lysate (p/n W07-000-357). Lane 4: MDA-MB-231 (p/n W09-001-GK6). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: Jurkat Lysate (p/n W09-001-370). Lane 7: NIH/3T3 Lysate (p/n W10-000-358). Lane 8: E-coli HCP Control (p/n 000-001-J08). Lane 9: FLAG Positive Control Lysate (p/n W00-001-383). Lane 10: Red Fluorescent Protein (p/n 000-001-379). Lane 11: Green Fluorescent Protein (p/n 000-001-215). Lane 12: Glutathione-S-Transferase Protein (p/n 000-001-200). Lane 13: Maltose Binding Protein (p/n 000-001-385). Load: 10 μg of lysate or 50ng of purified protein per lane. Primary antibody: GST antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody (p/n 605-4302) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 26 kDa for GST.
|
|
| 別品名 |
goat anti-GST antibody, Glutathione-S-Transferase
|
| 別包装 |
あり
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
|
| 免疫動物 |
Goat
|
| 標識物 |
Unlabeled
|
| 精製度 |
Affinity Purified
|
| Accession No.(Gene/Protein) |
P08515
|
| Tag情報 |
GST
|
| 参考文献 |
[[Cosmo Reference Collection]][Pub Med ID]25890248,30462291,25384973,22904036,26240167,32994219,22322860,23817741,33306155,29507658,26867008,35162997,34741085,37072777,36699658
|
|
| メーカー |
品番 |
包装 |
|
RKL
|
600-101-200
|
1 MG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
４℃
|
|
※当社では商品情報の適切な管理に努めておりますが、表示される法規制情報は最新でない可能性があります。
また法規制情報の表示が無いものは、必ずしも法規制に非該当であることを示すものではありません。
商品のお届け前に最新の製品法規制情報をお求めの際はこちらへお問い合わせください。
|
※当社取り扱いの試薬・機器製品および受託サービス・創薬支援サービス（納品物、解析データ等）は、研究用としてのみ販売しております。
人や動物の医療用・臨床診断用・食品用としては、使用しないように、十分ご注意ください。
法規制欄に体外診断用医薬品と記載のものは除きます。
|
|
※リンク先での文献等のダウンロードに際しましては、掲載元の規約遵守をお願いします。
|
|
※CAS Registry Numbers have not been verified by CAS and may be inaccurate.
|
