| 別品名 |
PhosphoTyrosine reactive antibody, monoclonal phosphotyrosine, anti-phosphotyrosine produced in mouse, Mouse anti-phosphotyrosine
|
| 標識物 |
Unlabeled
|
| 精製度 |
Ascites
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
|
| 免疫動物 |
Mouse
|
| 抗体クラス |
IgG1κ
|
| クローン |
13F9
|
| 翻訳後修飾 |
リン酸化
|
| 形状 |
滅菌済み液状品
|
| 参考文献 |
Sarmay, G., et al., (1994) Protein-tyrosine kinase activity tightly associated with human type II Fc gamma receptors. Proc. Natl. Acad. Sci. USA 91, 4140. Arad-Dann, H., (1993) Immunohistochemistry of phosphotyrosine residues: identification of distinct intracellular patterns in epithelial and steroidogenic tissues. J. Histochem. Cytochem. 41, 513. Dvir, A., et al., (1991) The inhibition of EGF-dependent proliferation of keratinocytes by tyrphostin tyrosine kinase blockers. J. Cell Biol. 113, 857.
|
|
※サムネイル画像をクリックすると拡大画像が表示されます。
ELISA of Mouse anti phosphotyrosine antibody. Antigen: BSA conjugates of pT, pY and pS. Coating amount: 0.1 ug per well. Primary antibody: Phosphotyrosine antibody at 1:1000. Dilution series (X axis): 2 fold. Secondary antibody: HRP conjugated Gt a Mouse IgG H&L secondary antibody at 1:20,000. Substrate: TMB (p/n TMBE 0100).
Western Blot of Mouse anti-phosphotyrosine antibody (clone 13F9). Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after Coomassie staining. Load: 10 ug per lane. Primary antibody: phosphotyrosine antibody at 1:1000 for 1 hr at RT. Secondary antibody: IRDyeTM800 Conjugated Goat-a-Mouse IgG [H&L] (610-132-120) diluted 1:2,500 for 30' at room temperature. Block: 5% BLOTTO overnight at 4C. Other band(s): phosphotyrosine splice variants and isoforms.
|
|
|
|
ELISA of Mouse anti phosphotyrosine antibody. Antigen: BSA conjugates of pT, pY and pS. Coating amount: 0.1 ug per well. Primary antibody: Phosphotyrosine antibody at 1:1000. Dilution series (X axis): 2 fold. Secondary antibody: HRP conjugated Gt a Mouse IgG H&L secondary antibody at 1:20,000. Substrate: TMB (p/n TMBE 0100).
|
|
|
| メーカー |
品番 |
包装 |
|
RKL
|
500-301-262
|
100 UL
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
|
|
