Anti TNF α  

Western Blot of Mouse TNFα Antibody. Lane 1: Mouse TNFα. Load: 50 ng per lane. Primary antibody: Mouse TNFα antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Mouse TNFα.

(n,o) Representative immunostaining images of TNF-α (n) and TGF-β (o) in lungs from either non-tumor-bearing mice or mice bearing 4T1 or C26m2 metastases at five weeks post tumor cell injection. Scale bars, 100 μm. (p,q) Immunoblot analysis of phosphorylated p65 and Smad2 in muscles after TNF-α or TGF-β neutralizing antibody treatment. Following the tumor-resection-and-relapse approach (see Fig. 1a and Supplementary Fig. 1c), mice bearing either 4T1 (p) or C26m2 (q) metastases were treated with either an isotype control antibody, or a neutralizing antibody against TNF-α, TGF-β, or both (200 μg antibody per mouse treated three times a week) starting one week after surgery, for a period of one week (see Fig. 3e,f). SF3. PMID:？29875463

TNFα as a surrogate marker of inflammation in brain and serum of mice. Mean TNFα chemiluminescence in brain and serum of htau (purple) and control C57BL/6 J (grey) mice as a function of treatment (striped bars = r‐irisin treated mice; solid bars = vehicle‐treated mice). (A) Treatment with r‐irisin significantly reduced elevated levels of pro‐inflammatory cytokine TNFα in hippocampus of female htau mice. TNFα in C57 control mice did not vary as a function of treatment. (B) Brainstem and prefrontal cortex ptau levels appeared to be reduced in irisin‐treated female htau mice, but this was not statistically significant. Scatterplots depict strong correlation (indicated by Pearson's？r？value,？p？< 0.05) between hippocampal TNFα and ptau measurements for individual female htau mice. (C) Mean serum measurements of TNFα were significantly reduced in irisin‐treated female htau mice. (D) Irisin‐treated male htau mice showed significantly enhanced hippocampal inflammation compared to vehicle‐treated htau mice. (E) TNFα also appeared to be elevated in irisin‐treated male htau prefrontal cortex data but this was not statistically significant. Scatterplots illustrate the lack of correlation between hippocampal TNFα and ptau measurements for individual male htau mice (p = ns). (F) Mean serum measurements of TNFα did not differ as a function of irisin treatment in male htau mice, however they trended towards an irisin‐induced increase. Results are calibrated by total protein loaded per sample. Error bars show s.e.m.; * indicates significant difference between treatment groups. # indicates significant strain difference between htau and C57 mice of same treatment group.？p？< 0.05 in all significant comparisons. n = 16 htau mice (4/sex/treatment group); 14 C57 mice (3‐4/sex/treatment group). Fig 3. PMID:？33768561