別品名 |
APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
IgG fraction
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適用 |
Western Blot Immunohistochemistry Immuno Fluorescence
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免疫動物 |
Rabbit
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交差種 |
Human
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GENE ID |
7124
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Accession No.(Gene/Protein) |
P01375
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Gene Symbol |
TNFA
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]29388058, 16687193, 20131287
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry using Rockland's polyclonal TNFa antibody showing staining of formalin/PFA fixed paraffin embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum.? Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4C.? HRP conjugated goat anti rabbit antibody was used as the secondary antibody.
Fluorescent immunohistochemistry showing staining of human colon by Rockland's anti-TNF alpha (formalin/PFA-fixed paraffin-embedded sections). Samples were formaldehyde-fixed, then blocked in 10% serum for 2 hours at 20C. The primary antibody was diluted 1:100 and incubated with the sample for 2 hours at 20C. Alexa FluorR 680 goat polyclonal secondary antibody was used diluted 1:5000.
Western Blot of Rabbit anti-TNF Alpha antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: A549 Lysate (p/n W09-001-372). Lane 7: LNCap Lysate (p/n W09-001-GJ9). Lane 8: MOLT-4 Lysate (p/n W09-001-GK2). Lane 9: Ramos Lysate (p/n W09-000-GK4). Lane 10: Raji Lysate (p/n W09-001-368). Lane 11: A-172 Lysate (p/n W09-001-GL5). Lane 12: NIH/3T3 Lysate (p/n W10-000-358). Load: 35 ug per lane. Primary antibody: TNF Alpha antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 26kDa for TNF Alpha.
Western blot using Rockland's Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T for 30 min at RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4C, DyLight 649 Gt-a-Rb (p/n 611-143-122) added at 1:20,000 in buffer (p/n MB-070) for 30 min at RT.
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Immunohistochemistry using Rockland's polyclonal TNFa antibody showing staining of formalin/PFA fixed paraffin embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum.? Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4C.? HRP conjugated goat anti rabbit antibody was used as the secondary antibody.
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メーカー |
品番 |
包装 |
RKL
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209-401-306
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1 MG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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