別品名 |
Cyclin-dependent kinase inhibitor 2A, isoform 3 p19ARF
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抗原部位 |
a.a.54-75
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種由来 |
Mouse
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標識物 |
Unlabeled
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精製度 |
Ig fraction - Protein G
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immuno Fluorescence
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免疫動物 |
Rat
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抗体クラス |
IgG2b
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クローン |
5.C3.1
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交差種 |
Human Mouse Hamster
|
GENE ID |
12578
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Accession No.(Gene/Protein) |
Q64364
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Gene Symbol |
CDKN2A
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]30080881
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot analysis is shown using Rockland's Protein G Purified Rat Monoclonal Anti p19Arf antibody (5.C3.1) to detect endogenous mouse p19Arf protein present in wtMEFp6 cells (wild type mouse embryo fibroblasts passage 6). No reactivity is observed in lysates from NIH3T3 cells, which lack the Arf gene. Approximately 50 ug of cell lysate was loaded on a SDS PAGE gel, blotted onto a PVDF membrane and probed with 1.0 mg/ml of rat anti p19Arf antibody (both clones 5 C3 1 and 12 A1 1 are shown). The blot was visualized using HRP conjugated Gt anti Rat IgG followed by ECL reagent. (See Bertwistle et al. 2004 for details.)
Immunofluorescence microscopy using Rockland's Protein G Purified Rat Monoclonal Anti-p19Arf antibody to detect endogenous mouse p19Arf protein present in sections of testis from a wild type mouse at stage P11, probed with 5-C3-1 MAb (green). The section was also incubated with DAPI, to show positions of nuclei (blue). A similar section from the testis of an Arf -/- mutant mouse does not stain with this MAb (data not shown). Tissue was perfused with 4% paraformaldehyde in PBS. Slides were placed in a jar containing citrate buffer at pH 6.0 at 96 C for 30 min followed by blocking in 10% normal goat serum in 0.1% Triton X-100-PBS for 30 min. Slides were incubated with 5 ug/ml antibody at 4 C overnight followed by additional of fluorochrome conjugated secondary antibody. See Bertwistle et al. 2004 for additional details.
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Western blot analysis is shown using Rockland's Protein G Purified Rat Monoclonal Anti p19Arf antibody (5.C3.1) to detect endogenous mouse p19Arf protein present in wtMEFp6 cells (wild type mouse embryo fibroblasts passage 6). No reactivity is observed in lysates from NIH3T3 cells, which lack the Arf gene. Approximately 50 ug of cell lysate was loaded on a SDS PAGE gel, blotted onto a PVDF membrane and probed with 1.0 mg/ml of rat anti p19Arf antibody (both clones 5 C3 1 and 12 A1 1 are shown). The blot was visualized using HRP conjugated Gt anti Rat IgG followed by ECL reagent. (See Bertwistle et al. 2004 for details.)
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メーカー |
品番 |
包装 |
RKL
|
200-501-891
|
100 UG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
保存温度 |
-20℃
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