Anti Streptavidin

Dot Blot of Rabbit anti Streptavidin Biotin Conjugated. Antigen: Lane 1 Biotin conjugated Streptavidin Antibody. Lane 2 Streptavidin Antibody. Load: 3 fold serial dilution starting at 200 ng. Secondary antibody: HRP Streptavidin at 1:40,000 for 60 min at RT. Block: 1% BSA TTBS 30 min at RT.

Western Blot of Rabbit Anti-Trypsin Inhibitor Antibody and Rabbit Anti-Streptavidin Antibody. Lane 1: Trypsin Inhibitor reduced. Lane 2: Trypsin Inhibitor non-reduced. Lane 3: Streptavidin reduced. Lane 4: Streptavidin non-reduced. Load: ~1ug per lane. Primary antibody: Primary antibody at 1:1000 for overnight at 4C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 18.8 kDa, ~15 kDa for Streptavidin, 24 kDa, ~20 kDa for Trypsin Inhibitor. Other band(s): none.