別品名 |
HRP
|
種由来 |
Streptomyces avidinii
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標識物 |
Horseradish Peroxidase
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精製度 |
Ig fraction - Ion Exchange /Gel Filtration
|
適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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交差種 |
Streptomyces avidinii
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添加剤 |
Azide Free
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参考文献 |
Bayer EA Wilchek M Protein biotinylation. Methods Enzymol 184:138-60 (1990).
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Western Blot Results of Rabbit Anti-Streptavidin Antibody Peroxidase Conjugated. Lane 1: Streptavidin Reduced [0.05μg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Streptavidin Non-Reduced [0.05μg]. Primary Antibody: Anti-Streptavidin Peroxidase at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase (p/n 611-103-122) at 1:70,000 for 30mins at RT. Blocking Buffer: BlockOutR Universal Blocking Buffer (p/n MB-073) for 1 hour at RT. Expect: Reduced- monomer ~17kDa; Non-Reduced- tetramer ~45kDa. Exposure: 5sec.
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Western Blot Results of Rabbit Anti-Streptavidin Antibody Peroxidase Conjugated. Lane 1: Streptavidin Reduced [0.05μg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Streptavidin Non-Reduced [0.05μg]. Primary Antibody: Anti-Streptavidin Peroxidase at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase (p/n 611-103-122) at 1:70,000 for 30mins at RT. Blocking Buffer: BlockOutR Universal Blocking Buffer (p/n MB-073) for 1 hour at RT. Expect: Reduced- monomer ~17kDa; Non-Reduced- tetramer ~45kDa. Exposure: 5sec.
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メーカー |
品番 |
包装 |
RKL
|
200-4395
|
20 MG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
保存温度 |
4℃
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