Anti TMBIM1  

Immunohistochemistry of Rabbit anti-TMBIM1 antibody. Tissue: human prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TMBIM1 antibody at 10 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TMBIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of Rabbit anti-TMBIM1 antibody. Tissue: human tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TMBIM1 antibody at 10 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TMBIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Western blot using Rockland's protein A purified anti-TMBIM1 antibody shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-hTMBIM1 (lane 1). No staining is observed in lysates from mock transformed HeLa cells (lane 2). To date this antibody has shown the ability to recognize over expressed TMBIM1 ~35kDa, but not endogenous protein. The membrane was probed with the primary antibody at a 1:1,000 dilution at 4° C, overnight. Personal Communication from Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.